China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (9): 3484-3491.doi: 10.16431/j.cnki.1671-7236.2022.09.022

• Genetics and Breeding • Previous Articles     Next Articles

Effects of Different Concentrations of Taurine on the Preservation of Boar Semen Under Negative Pressure at Room Temperature

WANG Hechuan, ZHANG Han, LI Tianfeng, WANG Qian, ZHANG Qun, GUO Minghui, SUN Chao, ZHOU Yanzhi, LI Yuan, LI Yanbing, WEI Guosheng, LI Jingchun   

  1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2022-04-07 Online:2022-09-05 Published:2022-08-24

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Abstract: 【Objective】 The aim of this study was to investigate the effect of adding different concentrations of taurine to Modena diluent under negative pressure on the quality and antioxidant capacity of Landrace boar sperm stored at room temperature, in order to provide reference for the improvement of pig semen preservation system.【Method】 Taurine was added to Modena diluent at the concentration of 0(control group), 1, 5 and 10 mmol/L, respectively, and the semen of each group were stored under the condition of-0.04 Mpa for 11 days.On 1st, 3rd, 5th, 7th, 9th and 11th days, sperm motility, viability, malformation rate, average path velocity (VAP), curvilinear velocity (VCL) and beatcross frequency (BCF) were detected by computer-aided sperm analysis system (CASA).The total antioxidant capacity (T-AOC) and H2O2 content of semen were determined by kits.【Result】 The motility and viability of boar sperm in the three taurine groups were significantly higher than those in control group during the storage period of 1-11 days (P<0.05).On the 11th day, the malformation rate of boar sperm in 5 mmol/L taurine group was significantly lower than that in other groups (P<0.05).From the 3rd day, the VAP of boar sperm in 1, 5 and 10 mmol/L taurine groups were significantly higher than those in control group (P<0.05), and the 5 mmol/L taurine group had the best effect.On the 11th day, the VCL and BCF of boar sperm in 5 mmol/L taurine group were significantly better than those in other groups (P<0.05).Taurine could significantly increase the T-AOC of boar sperm (P<0.05), but the T-AOC was almost undetectable on the 11th day.From 1 to 3 days, there was no significant difference in the H2O2 content of boar sperm among all test groups, from 5 to 11 days, the H2O2 content of the three taurine groups was significantly lower than that of the control group (P<0.05).【Conclusion】 Under the condition of -0.04 Mpa, adding taurine to Modena diluent could significantly improve the quality parameters and antioxidant properties of boar semen stored at room temperature, and the best concentration was 5 mmol/L, and the storage time was less than 9 days.

Key words: negative pressure; taurine; boar semen; room temperature preservation

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