添加咖啡因、亚牛磺酸对牛精子功能的影响

doi:10.16431/j.cnki.1671-7236.2018.08.029

Abstract

Abstract:

The effect of caffeine and hypotaurine were evaluated on sperm function of bovine in this study.Frozen-thawed semen from Holstein bulls was used in sperm capacitation media with different concentrations caffeine (0,2.5,5.0 and 7.5 mmol/L) or hypotaurine (0,5,10,20 and 40 μmol/L),200 μL semen for each treatment was submitted to swim-up for 45 min in order to evaluate the effect of caffeine and hypotaurine on bovine sperm motility,acrosome integrity and plasma membrane integrity.Furthermore,the effect of hypotaurine as substitutes for caffeine in sperm capacitation media was investigated.The results showed that compared with control group,2.5 and 5.0 mmol/L caffeine resulted in greater sperm motility and the rate of acrosome integrity(P<0.05),the greatest sperm motility was observed with 2.5 mmol/L caffeine.The sperm motility,the rate of acrosome integrity and plasma membrane integrity of 10 and 20 μmol/L hypotaurine were significantly higher than those of control group (P<0.05),20 μmol/L hypotaurine was the best on sperm function parameters.The effect of 2.5 mmol/L caffeine and 20 μmol/L hypotaurine on sperm function were evaluated.Compared with 2.5 mmol/L caffeine and control group,20 μmol/L hypotaurine resulted in greater the rate of acrosome integrity and plasma membrane integrity (P<0.05).In summary,2.5 mmol/L caffeine might be replaced by 20 μmol/L hypotaurine in sperm capacitation media,it was beneficial to improve sperm function parameters.

Key words: hypotaurine; caffeine; bovine; sperm function

CLC Number:

S814.4

LI Xiaoxia, CAO Pinghua, YU Xueli, LI Yinghua. Effect of Caffeine and Hypotaurine on Sperm Function of Bovine[J]. , 2018, 45(8): 2276-2281.

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Effect of Caffeine and Hypotaurine on Sperm Function of Bovine

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