China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (2): 606-615.doi: 10.16431/j.cnki.1671-7236.2023.02.018

• Genetics and Breeding • Previous Articles     Next Articles

The Study on the Role of Fetal Bovine Serum Supplementation During Postwarming Culture on Vitrified Porcine Parthenogenetic Blastocysts

XIANG Decai1, LI Shuiying1,2, ZHANG Bin1, ZHANG Yan1, LIANG Jiachong1, LYU Chunrong1, HONG Qionghua1, QUAN Guobo1, WU Guoquan1   

  1. 1. National Regional Genebank (Yunnan) of Livestock and Poultry Genetic Resources, Yunnan Animal Science and Veterinary Institute, Kunming 650224, China;
    2. Agricultural and Rural Science and Technology Bureau of Honghe County, Honghe Hani and Yi Autonomous Prefecture 654499, China
  • Received:2022-08-02 Online:2023-02-05 Published:2023-02-06

Abstract: 【Objective】 The aim of this study was to evaluate the effects of fetal bovine serum (FBS) supplementation during postwarming culture on vitrified porcine parthenogenetic blastocysts.【Method】 The porcine parthenogenetic blastocysts on the 5th day of in vitro culture were used in this experiment,and the fresh and vitrified blastocysts were cultured in the embryo culture medium containing 10% FBS (V/V) for 48 h.This experiment was divided into four groups:Fresh,Fresh+FBS,Vitrified and Vitrified+FBS groups.The blastocysts expansion and hatching rates,the percentages of membrane damage and apoptosis and total cell number were determined.Moreover,the intracellular reactive oxygen species (ROS) levels,mitochondrial activity and expression levels of genes related embryo development in the blastocysts were detected.【Result】 Compared with the Fresh and Vitrified groups, the complete expansion rate, hatching rate and total number of blastocyst cells in the Fresh+FBS and Vitrified+FBS groups were significantly increased (P<0.05), while the cell membrane damage rate and apoptotic cell rate were significantly decreased (P<0.05). Compared with the Fresh group, ROS levels were significantly increased in the Vitrified group, while ROS levels were significantly decreased in both the Fresh+FBS and Vitrified+FBS groups (P<0.05). The mitochondrial activity of Vitrified+FBS group was significantly higher than that of Vitrified+FBS group and significantly lower than that of Fresh+FBS group (P<0.05), but there was no significant difference between Vitrified+FBS group and Fresh group (P>0.05). Compared with the Fresh group, POU class 5 homeobox 1(POU5F1) gene expression level in Vitrified group was significantly increased (P<0.05) and catalase (CAT)gene expression level was significantly decreased (P<0.05). There were no significant differences in the expression levels of proliferating cell nuclear antigen (PCNA), DNA methyltransferase 3A (DNMT3A), superoxide dismutase 1 (SOD1) and BCL2-associated X protein (BAX):BCL2L1 between the Fresh and Vitrified groups (P>0.05). The expression levels of PCNA, SOD1 and CAT genes in Fresh+FBS and Vitrified+FBS groups were significantly higher than those in Fresh and Vitrified+FBS groups (P<0.05).【Conclusion】 This study found that fresh and vitrified parthenogenes blastocysts were cultured in 10% FBS on the 5th day of in vitro culture, and their development ability and quality of embryo were significant improved.

Key words: pig; blastocyst; vitrification; fetal bovine serum; embryo quality

CLC Number: