培养组学在动物肠道微生物应用的研究进展

doi:10.16431/j.cnki.1671-7236.2023.02.017

Abstract

Abstract: Intestinal microorganisms,which is called the 'hidden immune organ’ of animals and is crucial to maintain body health,can not only participate in host metabolism,but also affect the host’s immune system.The author mainly introduced the development history of culturomics and its significance to the study of animal intestinal microorganisms,and the advantages and disadvantages of traditional microbial culture methods and molecular biology methods in the study of microorganisms. The culturomics is a new method for the isolation and identification of microorganisms which is based on traditional microbial culture methods and simultaneously uses multiple culture conditions to culture microorganisms,supplemented by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA (16S ribosomal RNA) gene sequencing technology to isolate and identify microorganisms.This method combines the advantages of traditional microbial culture technology and molecular biology technology.This method has the advantage of discovering,finding,and obtaining in the study of excavating 'new microorganisms’.In microbial studies,target strains can be customized for verification and the gut microbiome can be clearly understood through rich annotations.In addition,the author analyzes the research and application of culturomics in the intestines of poultry,pigs,ruminants and other animals,and puts forward the impact of environmental conditions on intestinal microorganisms,such as the impact of human contact on intestinal flora,and the differences between intestinal flora of the same species and different genders,hoping to provide reference for the research and application of culturomics in animal intestinal microorganisms.

Key words: animal gut microbiota; culturomics; digestive tract; molecular biology

CLC Number:

Q93-31

QIU Fei, HAN Yixin, WEI Qilin, TANG Xinggang, YUAN Minggui, DONG Bo, XIANG Rong, XU Zhihong. Research Progress of Culturomics Application in Animal Gut Microbes[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 598-605.

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Research Progress of Culturomics Application in Animal Gut Microbes

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