冷冻稀释液中添加大豆卵磷脂对梅花鹿冻融精子质量的影响

doi:10.16431/j.cnki.1671-7236.2022.11.023

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of adding soybean lecithin instead of 10% egg yolk to the frozen diluent on the cryopreservation of semen of sika deer, in order to provide references for the improvement of the artificial insemination system of sika deer.【Method】 The semen of sika deer was collected by electrical stimulation, and 1%, 2%, 3%, 4% and 5% soybean lecithin was added to the frozen diluent to replace 10% egg yolk as the experimental group, and 20% egg yolk was added as control group, respectively.Cryopreservation of semen in each group was performed.After 5 days, the semen was thawed, and the sperm motility, plasma membrane integrity rate, acrosome integrity rate, mitochondrial activity, and survival time of each group were detected after thawing, and soybean lecithin of suitable concentration was screened.Healthy female sika deer aged 4-5 years were selected, intramuscularly injected with 300 IU PMSG and 0.4 mg cloprostol sodium for simultaneous estrus treatment, and artificial insemination was performed with frozen semen in the 20% egg yolk group and the selected soybean lecithin group at 20 h after estrus, 30 days after insemination, B-ultrasound detector was used to detect the pregnancy, and count the pregnancy rate.【Result】 Compared with control group, the sperm motility, forward motility, fast-forward motility, motility, plasma membrane integrity rate, acrosome integrity rate and mitochondrial activity were significantly improved in 1% soybean lecithin group after freezing and thawing (P<0.05).With the increase of the concentration of soybean lecithin in the diluent, the sperm motility, forward motility, fast forward motility, motility, plasma membrane integrity rate, acrosome integrity rate and mitochondrial activity showed a downward trend, and sperm survival time also decreased with the increasing concentration.The pregnancy rate of sika deer in 1% soybean lecithin group was 61.11% after artificial insemination of frozen-thawed sperm, which was higher than that of control group, 2% and 3% soybean lecithin groups, but the difference was not significant (P>0.05).【Conclusion】 Adding 1% soybean lecithin to replace 10% egg yolk in the frozen diluent of sika deer sperm could effectively improve the quality of the frozen and thawed semen of sika deer, and provide a theoretical basis for further screening of new frozen diluents of sika deer semen.

Key words: sika deer; soybean lecithin; sperm; cryopreservation; egg yolk

CLC Number:

S825

LI Mengjie, ZHAO Weigang, GUO Xiaolan, GU Shigang, ZHAO Quanmin, XU Chao. Effects of Soybean Lecithin in Frozen Diluent on Quality of Frozen-thawed Sperm of Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4327-4334.

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Effects of Soybean Lecithin in Frozen Diluent on Quality of Frozen-thawed Sperm of Sika Deer

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