程序化冷冻前后小鼠正常孵化囊胚和休眠胚胎中C3基因表达的研究

doi:10.16431/j.cnki.1671-7236.2017.02.030

Abstract

Abstract:

The study was aimed to investigate the protein location and the transcriptional expression of C3 on mouse normal hatched blastocysts and dormant embryos before and after cryopreservation, and to provide theoretical basis for elucidating the regulation mechanism of mammalian embryos during antifreeze. The ICR female mouse were selected, normal hatched blastocysts were collected from 5th pregnant mouse and dormant embryos were obtained from mouse delayed implantation model, then the confocal and Real-time PCR were used to detect ICC and relative expression of C3 mRNA on each group. The results showed that C3 were both expressed on transcriptional and translational levels in mouse normal hatched blastocysts and dormant embryos before and after cryopreservation;The relative expression of C3 mRNA in normal hatched blastocysts was significantly up-regulated after cryopreservation (P < 0.05);The relative expression of C3 mRNA in dormant embryos was significantly down-regulated after cryopreservation (P < 0.05);The relative expression of C3 mRNA in dormant embryos before cryopreservation was significantly higher than the normal hatched blastocysts before cryopreservation (P < 0.05);The relative expression of C3 mRNA in dormant embryos after cryopreservation was significantly lower than the normal hatched blastocysts after cryopreservation (P < 0.05). The results showed that the transcriptional down-regulated expression of C3 in embryos had a positive effect on the antifreeze of embryos, and C3 gene might be involved in the regulation of embryo freezing-thawing process.

Key words: mouse; blastocysts; dormant embryo; cryopreservation; C3 gene

CLC Number:

S814.8

LIU Di, NI He-min, GU Mei-chao, LU Wen-jin, SHENG Xi-hui, QI Xiao-long, WANG Xiang-guo, GUO Yong. Study on the Expression of C3 Gene on Mouse Normal Hatched Blastocysts and Dormant Embryos before and after Cryopreservation[J]. , 2017, 44(2): 514-520.

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Study on the Expression of C3 Gene on Mouse Normal Hatched Blastocysts and Dormant Embryos before and after Cryopreservation

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