螺旋藻抗炎和免疫增强作用的研究

doi:10.16431/j.cnki.1671-7236.2019.07.031

Abstract

Abstract:

The aim of this experiment was to evaluate the anti-inflammatory effect and immune effect of Spirulina.An inflammation model of mice was constructed by xylene induced mice ear swelling,and the effect of Spirulina on anti-inflammatory was investigated by using dexamethasone in positive control group and mice ear swelling as observation indexes.An immune-suppression model of mice was set up with cyclophosphamide,and the immune effect of Spirulina was investigated by comparing the organ indexes,levels of IL-2,IL-6,IL-1β,TNF-α and IFN-γ,and spleen and thymus histopathology in both normal and immune-suppressed mice treated with different doses of Spirulina.The results showed that:In the anti-inflammatory experiment,the ear swelling inhibition rate of mice treated with 0.3% spirulina was extremely significantly higher than that of dexamethasone group and other Spirulina treatmens (P<0.01),and viscera index of all Spirulina groups had no significant difference (P>0.05);In the immune function experiment,compared with blank control group,the spleen index and thymus index were extremely significantly decreased (P<0.01),and the liver index was extremely significantly increased (P<0.01) in cyclophosphamide positive control group,and the thymus indexes of Spirulina treated groups had no significant difference (P>0.05).Serum IL-2,IL-6,TNF-α and IFN-γ levels had no significant difference among all the groups (P>0.05).At the same time,the decrease of thymus corpuscles and the degeneration and necrosis of lymphocytes and reticular cells were observed in the positive control group by histopathological observation,while the structure of spleen corpuscles and thymus corpuscles in Spirulina treatments were more clear and complete,and the lymphocytes were increased.In conclusion,Spirulina could reduce the immunosuppression of dexamethasone and cyclophosphamide on mice,and repair the damage of spleen and thymus in mice.Spirulina played a certain role in anti-inflammatory and alleviating immunosuppression.

Key words: Spirulina; mouse; anti-inflammatory; immune function

CLC Number:

R961

CHEN Zhongwei, QIU Jie, LI Xiaoyu, ZHAO Wu, QIN Yibin, LU Bingxia, DUAN Qunpeng, LIANG Jiaxing, LI Bin, ZHOU Yingning, HU Tingjun, HE Ying. Study on Anti-inflammatory and Immune Effect of Spirulina[J]. , 2019, 46(7): 2135-2143.

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Study on Anti-inflammatory and Immune Effect of Spirulina

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