硒对铅致小鼠睾丸间质细胞凋亡的影响

doi:10.16431/j.cnki.1671-7236.2023.04.012

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of selenium on lead-induced apoptosis in mouse Leydig cells in vitro.【Method】 Mouse Leydig cells were divided into control,selenium,lead and selenium＋lead groups.2 μmol/L sodium selenite was added into the cell culture medium of selenium group,40 μmol/L lead acetate was added into the cell culture medium of lead group,2 μmol/L sodium selenite and 40 μmol/L lead acetate were added into the cell culture medium of selenium＋lead group.The cells were cultured for 24 h in vitro.Cell proliferation was detected by MTT assay.The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in cells were detected by biochemical analysis.Reactive oxygen species (ROS) level was measured by chemical fluorescence.Real time quantitative PCR and Western blotting were used to detect the mRNA and protein expression levels of cysteinyl aspartate specific proteinase 3 (Caspase3),cysteinyl aspartate specific proteinase 8 (Caspase8),B-cell lymphoma-2 (Bcl-2) and Bcl2-associated X (Bax).【Result】 Compared with control group, cell proliferation was significantly inhibited in lead group (P＜0.05),the content of MDA and ROS level were significantly increased (P＜0.05),and the activities of SOD and GSH-Px were significantly decreased (P＜0.05).At the same time,lead significantly up-regulated the mRNA and protein levels of Caspase3,Caspase8 and Bax (P＜0.05),but down-regulated the mRNA and protein levels of Bcl-2 (P＜0.05).The proliferative activity of Leydig cells in selenium group was significantly increased than that in control group (P＜0.05).Compared with lead group,the content of MDA and ROS level in the cells of the selenium＋lead group were significantly decreased,and the activities of SOD and GSH-Px were significantly increased,which were between control and lead groups (P＜0.05).The mRNA and protein expressions of Caspase3,Caspase8,and Bax in selenium＋lead group were significantly lower than those in lead group (P＜0.05),while Bcl-2 showed an opposite trend (P＜0.05).【Conclusion】 Lead could inhibit the proliferative activity of mouse Leydig cells in vitro and induce oxidative stress and apoptosis.Supplementing appropriate amount of selenium could promote cell proliferation,alleviate lead-induced cell oxidative stress and apoptosis by increasing the activity of antioxidant enzymes.

Key words: selenium; lead; mouse; Leydig cells; apoptosis

CLC Number:

S814

GUO Yaru, LI Kexin, WANG Li, LIU Jinyu, SHI Lei, REN Youshe. Effects of Selenium on Lead-Induced Apoptosis of Mouse Leydig Cells in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1404-1411.

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Effects of Selenium on Lead-Induced Apoptosis of Mouse Leydig Cells in vitro

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