miR-188对小鼠乳腺癌细胞增殖、迁移及凋亡的影响

doi:10.16431/j.cnki.1671-7236.2022.03.038

Abstract

Abstract: 【Objective】 This study was aimed to investigate the regulatory role of microRNA-188-5p (miR-188) on the proliferation, migration and apoptosis of breast cancer cells, in order to provide a theoretical basis for the development of breast cancer related treatment drugs.【Method】 The mouse breast cancer cells (4T1) was cultured to establish 4T1 mouse xenograft model.The tumour tissues and adjacent tissues were then separated, and the expression of miR-188 was detected by Real-time quantitative PCR.The oligos miR-188 mimics, mimics-NC, miR-188 inhibitor and inhibitor-NC were transferred into 4T1 cell lines, and the non transfected cells was blank control (Con), the expression level of miRNA-188 was detected by Real-time quantitative PCR, cell proliferation was detected by CCK-8 method, cell migration was detected by cell scratch test, the apoptosis rate was detected by flow cytometry, the expression of apoptosis related proteins was detected by Western blotting.【Result】 The expression of miR-188 in peritumoral tissues was significantly higher than that of tumour tissues (P<0.05).The results of cell transfection showed that compared with Con group, the relative expression of miR-188 in miR-188 mimics group was significantly up-regulated (P<0.05), and in miR-188 inhibitor group was significantly down regulated (P<0.05).There was no significant difference between mimics-NC and inhibitor-NC groups.Therefore, the follow-up tests were analyzed with mimics-NC and inhibitor-NC as the control of miR-188 mimics and miR-188 inhibitor, respectively.Cell proliferation test and cell scratch test showed that compared with mimics-NC group miR-188 significantly reduced the proliferation rate and migration rate of 4T1 cells (P<0.05).Cell apoptosis test results showed that compared with mimics-NC group miR-188 mimics significantly promoted 4T1 apoptosis (P<0.05);Compared with inhibitor-NC group, miR-188 inhibitor significantly inhibited apoptosis (P<0.05).Western blotting results showed that miR-188 mimics significantly decreased the expression of matrix metalloproteinase-9 (MMP-9) protein (P<0.05), significantly increased the expression of adenosine diphosphate ribose polymerase (PARP1), cysteine protease-3 (caspase-3) and Bcl-2-associated X protein (Bax), and significantly inhibited the expression of anti-apoptotic protein B lymphoma-2 (Bcl-2) (P<0.05).【Conclusion】 miR-188 could significantly inhibit the proliferation and migration of 4T1 cells and promote the apoptosis of 4T1 cells, indicating that miR-188 could be used as a tumor regulator to inhibit the progression and metastasis potential of breast cancer.

Key words: breast cancer; 4T1 cells; miR-188; proliferation; apoptosis

CLC Number:

Q527

ZHU Xinying, GUO Shuai, ZHAN Xiaoyan, ZHONG Dengke, ZHENG Jiangping, HUA Zhimin, LI Tianshun. Effects of miR-188 on the Proliferation, Migration and Apoptosis of Mouse Breast Cancer Cells[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1153-1161.

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Effects of miR-188 on the Proliferation, Migration and Apoptosis of Mouse Breast Cancer Cells

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