清肺颗粒固态发酵培养基成分研究

doi:10.16431/j.cnki.1671-7236.2022.03.039

Abstract

Abstract: 【Objective】 The purpose of the experiment was to screen the best kinds of carbon source, nitrogen source and inorganic salt of Qingfei granule solid-state fermentation medium, optimize the composition of Qingfei fermentation medium and improve the content of epigoitrin in the formula.【Method】 A high performance liquid chromatography (HPLC) method was established for the determination of epigoitrin in fermented products.The carbon source (glucose, sucrose, mannitol, maltose, corn flour, soluble starch and herb), nitrogen source (peptone, soybean powder, bran, yeast extract, ammonium sulfate, ammonium chloride and urea) and inorganic salt(calcium carbonate, potassium dihydrogen phosphate, magnesium sulfate, manganese sulfate and sodium chloride) components of Qingfei granule solid-state fermentation medium were screened by single factor test.The content of epigoitrin in each gram of solid-state fermentation medium was determined by HPLC.Four factors (carbon source, nitrogen source, inorganic salt and water) and three levels response surface analysis was used to optimize the proportion of each medium component.The selected optimal medium component was used to replace the corresponding substances in the basic medium.The fermentation bacterium Bacillus amyloliquefacines was inoculated and cultured at 37 ℃ and 130 r/min for 22 h.Then the content of each component of the medium was analyzed by response surface analysis with the content of epigoitrin in each gram of solid fermentation culture as the evaluation index.【Result】 The optimum solid-state fermentation medium for Qingfei granules was traditional Chinese medicine powder, inorganic salt was calcium carbonate, and nitrogen source was soybean powder.The content of each medium optimized by response surface analysis was 30% traditional Chinese medicine powder, 10% of soybean powder, 0.2% of CaCO₃ and 59.8% of water.At this time, the content of epigoitrin in solid-state fermentation was 0.0184 mg/g.However, the content of epigoitrin in the prescription of Qingfei granules before optimization was 0.0150 mg/g, with extremely significant difference (P<0.01), it indicated that the fermentation process of Bacillus amyloliquefaciens was more conducive to the release of effective components in the prescription of Qingfei granules.【Conclusion】 The optimized solid-state fermentation substrate was easy for the growth of Bacillus amyloiquefaciens.

Key words: Qingfei granule; solid-state fermentation; culture medium; epigoitrin

CLC Number:

S853.7

ZHANG Yan, XUE Zhanmei, LIU Xuesong, SU Jing, HAO Jingyou, TANG Wei, LIU Yankai, SUN Hongjie, SHI Tongrui. Study on Components of Solid-state Fermentation Medium of Qingfei Granules[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1162-1171.

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Study on Components of Solid-state Fermentation Medium of Qingfei Granules

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