布氏乳杆菌、黑曲霉、热带假丝酵母、枯草芽孢杆菌与植物乳杆菌3菌组合对木薯渣发酵品质的影响

doi:10.16431/j.cnki.1671-7236.2016.07.011

Abstract

Abstract:

The experiment was carried out to investigate effects of three strains combinations of Lactobasillus buchneri (LAB),Aspergillus niger (AN),Candida tropicalis (CT),Bacillus subtilis (BS) and Lactobacillus plantarum (LAP) on fermentation quality of cassava residue.In this study,cassava residue were used as fermentative raw material,three strains were combined by 1∶1∶1 chosen from LAB,AN,CT,BS and LAP,getting five different combination.Combination 1:LAB+AN+LAP;Combination 2:LAB+CT+LAP;Combination 3:LAB+BS+LAP;Combination 4:AN+CT+LAP;Combination 5:CT+BS+LAP;1% urea or 1% urea+0.6% brown sugar was added to each combination;Blank group was without any additives;1% urea was added to control group Ⅰ and 1% urea+0.6% brown sugar were added to control groupⅡ.Experimental group Ⅰ was consisted of control group Ⅰ+different combination (1-5) while experimental group Ⅱ was consisted of control group Ⅱ+different combination (1-5).Water content in each group was about 65% by modulating with normal saline,each group was fermented in the vacuum of polyethylene film bag for 10 days.The results showed as follows:①pH of each experimental group was significantly lower than that of blank group and control group (P < 0.05),experimental group Ⅱ1 (LAB+AN+LAP+urea+brown sugar) could improve acetic acid content of cassava residue and reduce the NDF and ADF contents,and which were the lowest and were significantly lower than that of blank group and the control groups (P < 0.05);②Experimental group Ⅱ5 (CT+BS+LAP+urea+brown sugar) could improve the crude protein content in the cassava residue which was significantly higher than that of blank group and the control groups (P < 0.05);③It was favorable to propionic acid production by adding the corresponding strains+urea+brown sugar to the cassava residue fermentation;④Urea and brown sugar were added into cassava residue for improving the nutrition composition during the fermentation process was better than that only urea was added;⑤When the urea was added into the fermentation medium,the crude protein content in cassava residue was significantly increased,when cassava residue fermented quality was evaluate by butyric acid and acetic acid contents,all the groups got to the top of grade.In conclusion,solid-state fermentation using LAB+AN+LAP+urea+brown sugar or CT+BS+LAP+urea+brown sugar could offer an effective alternative to improve the fermentation quality of cassava residue.

Key words: cassava residue; mixed microbe strains; solid-state fermentation; quality evaluation

CLC Number:

S816

TANG Qing-feng, PENG Kai-ping, WEI Sheng-ju, ZOU Cai-xia, LIANG Xin, LI Li-li, HUANG Feng, LIN Bo, YANG Cheng-jian. Effects of Three Strains Combinations of Lactobasillus buchneri, Aspergillus niger, Candida tropicalis, Bacillus subtilis and Lactobacillus plantarum on Fermentation Quality of Cassava Residue[J]. , 2016, 43(7): 1729-1736.

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Effects of Three Strains Combinations of Lactobasillus buchneri, Aspergillus niger, Candida tropicalis, Bacillus subtilis and Lactobacillus plantarum on Fermentation Quality of Cassava Residue

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