›› 2016, Vol. 43 ›› Issue (9): 2302-2309.doi: 10.16431/j.cnki.1671-7236.2016.09.013

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Study on Formula Screening and Mixing Technology of Microbial Solid-state Fermentation Feed for Mutton Sheep

ZHANG Li-ming, GUO Peng, ZHANG Zheng, ZHANG Qi-wei, LIU Da-cheng   

  1. Key Laboratory of Animal Disease Clinical Diagnosis Technology, Ministry Department of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Received:2016-03-11 Online:2016-09-20 Published:2016-09-20

Abstract:

The experiment was designed to develop a microorganism fermentation feed for mutton sheep,and determined its solid-state mixing technology.In this experiment,the wheat bran,rice bran and jujube fruit residues were chosen as the solid-state fermentation media,and combined the Saccharomyces cerevisiae strains BC,XR4 and Bacillus subtilis strain A15 as the fermentation strains.The DPS software was applied to design the uniform mixture experiment,and 8 formulas were selected for solid-state fermentation experiments,and evaluated the optimal formula by measuring the number of live bacterium and three kinds of nutritional active substances (β-glucan,mannan and peptide).Meanwhile,we studied the mixing technology on the selected formula,and the control group was without turned mixing,the experimental group 1 was turned mixing once and the experimental group 2 was turned mixing twice.The optimal mixing technology was determined by monitoring the feed temperature,the number of live bacterium and the content of three kinds of nutritional active substances (β-glucan,mannan and peptides) during the solid-state fermentation.The results showed that the number of live bacterium of 8 formulas all increased first and then decreased rapidly.Three kinds of nutritional active substances were significantly increased with the extension of fermentation (P<0.05).The formula 7 had the optimal comprehensive index,of which the living bacterium number reached 37.30×105 CFU/g and the contents of three nutrient active substances were 97.41 mg/100 mg,37.66 mg/100 mg and 20.17 μg/100 mg.In the mixing technology experiment,the number of live bacterium and nutritional active substances of the experimental groups were significantly higher than the control group (P<0.05).Comparing with the control group,the number of live bacterium and the content of nutritional active substances in experimental group 2 increased by 10.1%,7.5%,7.6%,3.0%,respectively.

Key words: mutton sheep; microbial solid-state fermentation feed; feed formula; material mixing technology

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