不同产羔数绵羊性腺轴比较转录组研究

doi:10.16431/j.cnki.1671-7236.2017.02.027

›› 2017, Vol. 44 ›› Issue (2): 488-496.doi: 10.16431/j.cnki.1671-7236.2017.02.027

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Comparative Transcriptome Research of Gonadal Axis for Different Lambing Number of Sheep

FU Shao-yin^1,2,3, HE Xiao-long¹, WANG Biao¹, LI Hu-shan⁴, CHU Ming-xing⁵, LIU Yong-bin^1,3, ZHANG Wen-guang^2,3

1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
2. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
3. Institute of ATCG, Nei Mongolia Bio-information, Hohhot 010020, China;
4. Bayannur Livestock Improvement Station, Bayannur 015000, China;
5. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2016-07-07 Online:2017-02-20 Published:2017-02-25

Abstract

Abstract:

In order to obtain the important functional genes related to prolificacy,transcriptome of gonadal axis 5 Bamei mutton with high repeoduction and 4 Bamei mutton with low reproduction were analyzed using RNA-Seq technology. The sequencing data was analyzed by bioinformatics methods. After quality control, 112 Gb clean data were obtained from 27 libraries of hypothalamus, pituitary and ovary. The differential expression analysis underlined 111 genes highly expressed in the hypothalamus, 97 in the pituitary and 182 in the ovary between twinning group and single lambs;106 genes lower expression in the hypothalamus,142 in the pituitary and 67 in the ovary between twinning group and single lambs. Functional enrichment analysis revealed that neuroactive ligand-receptor interaction signaling pathway played an important role in the hypothalamic-pituitary-ovarian axis regulation of ovulation and follicular development. Comparative transcriptomic studies for different lambing number Bamei mutton sheep gonadal axis prodeced all transcript information, provided a list of functiona genes for influencing litter size, enriched and supplemented the sheep genome information, laid the theoretical basis for further elucidation the molecular mechanism of sheep fecundity differences.

Key words: Bamei mutton sheep; gonadal axis; comparative transcriptome

CLC Number:

S814.8

FU Shao-yin, HE Xiao-long, WANG Biao, LI Hu-shan, CHU Ming-xing, LIU Yong-bin, ZHANG Wen-guang. Comparative Transcriptome Research of Gonadal Axis for Different Lambing Number of Sheep[J]. , 2017, 44(2): 488-496.

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Comparative Transcriptome Research of Gonadal Axis for Different Lambing Number of Sheep

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