基于RNA-Seq技术挖掘绵羊背最长肌肉质性状相关基因

doi:10.16431/j.cnki.1671-7236.2018.01.016

Abstract

Abstract:

In order to obtain the genes related to meat quality traits, the transcriptome of longissimus dorsi of 3 Small-tail Han sheep and 3 Bamei mutton sheep were analyzed using RNA-Seq technology. The sequencing data was analyzed by bioinformatics methods then obtained the interrelated candidate genes which affect the meat quality traits. After quality control, 120 Gb clean data were obtained from 6 libraries. The differential expression gene were 333, which the expression of 82 genes was higher and 251 genes was lower. The results showed that 6 candidate genes (PDK4, MYF6, PPARGC1A, SLCO4A1, FABP4 and LEP) related to meat quality traits were screened by GO functional enrichment and KEGG Pathway analysis. Through comparison of the longissimus dorsi transcriptome data of Small-tail Han sheep and Bamei mutton sheep, we obtained the genes of meat quality traits, and enriched the information of sheep genome. This results laid a foundation for further elaboration of the molecular mechanism of meat quality traits in different sheep breeds.

Key words: RNA-Seq; meat quality trait; transcriptome; Small-tail Han sheep; Bamei mutton sheep; longissimus dorsi

CLC Number:

S813.3

WANG Wei, FU Shaoyin, HE Xiaolong, WANG Yanxin, WANG Yuexing, WANG Biao, LIU Bin, LIU Yongbin, ZHANG Wenguang. Excavation of Meat Quality Related Genes in Longissimus Dorsi of Sheep by RNA-Seq[J]. , 2018, 45(1): 122-130.

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Excavation of Meat Quality Related Genes in Longissimus Dorsi of Sheep by RNA-Seq

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