Abstract: 【Objective】 This study was aimed to explore the effect of selenized garlic polysaccharide on the function of mouse peritoneal macrophages, in order to provide a basis for the mining and clinical application of selenium garlic polysaccharide (sGPS).【Method】 The garlic polysaccharide(GPS) was obtained by isolation and purification, and modified by HNO₃-Na₂SeO₃ to obtain sGPS₃, GPS₅ and sGPS₆.Taking the mouse peritoneal macrophages as the research object, the mouse peritoneal macrophages were treated with sGPS₃, GPS₅, sGPS₆ at five concentrations (6.25, 12.5, 25, 50 and 100 μg/mL)and 10 μg/mL lipopolysaccharide (LPS group) for 48 h, at the same time, the cells without drugs were set as control group. The neutral red method was used to determine the phagocytic function of mouse peritoneal macrophages, and the CCK-8 method was used to determine the proliferation, in order to screen out sGPS with the best activity. The ELISA method was used to determine the effect of sGPS with the best activity on the contents of NO, TNF-α, IFN-γ, IL-1β, IL-6, and IL-12 in the supernatant of macrophages. The mouse peritoneal macrophages swallow chicken red blood cells and mouse carbon clearance experiments were used to detemine the phagocytic indexes and phagocytic percentage of blank control group (normal saline), high (2 mg/mL), medium (1 mg/mL) and low (0.5 mg/mL) doses of sGPS with the best activity.【Result】 Except 6.25 μg/mL sGPS₃, the A_{490 nm} values of 6.25, 12.5, 25, 50 and 100 μg/mL peritoneal macrophages in sGPS₃, sGPS₅ and sGPS₆ groups were significantly higher than those in control group (P<0.05).Among them the A_{490 nm} values 50 and 100 μg/mL of sGPS₆ groups were significantly higher than that of the LPS group (P<0.05).Therefore, sGPS₆ was selected for follow-up test.The contents of NO, TNF-α, IL-1β and IL-6 in the supernatant of mouse peritoneal macrophages of sGPS₆ at the concentrations of 12.5, 25, 50 and 100 μg/mL were significantly higher than those in control group (P<0.05).The contents of IFN-γ, IL-12 in the supernatant of mouse peritoneal macrophages of sGPS₆ at the concentrations of 25, 50 and 100 μg/mL were significantly higher than those in control group (P<0.05).Among them, the contents of NO, TNF-α and IL-12 of mouse peritoneal macrophage supernatant at 100 μg/mL of sGPS₆ were significantly higher than the LPS group (P<0.05).The phagocytic index and phagocytic rate at 2 and 1 mg/mL sGPS6 were significantly higher than those of the blank control group (P<0.05).【Conclusion】 Selenium garlic polysaccharide could enhance the phagocytic function and proliferation ability of the mouse peritoneal macrophages, and promote the secretion of cytokines TNF-α, IL-1β and IL-6.

Key words: selenized garlic polysaccharide; mouse peritoneal macrophages; phagocytic function; cytokine; phagocytic index