金黄色葡菌球菌脂蛋白对M1型小鼠骨髓源巨噬细胞免疫作用的影响

doi:10.16431/j.cnki.1671-7236.2021.08.041

Abstract

Abstract: The aim of this study was to clarify the effect of Staphylococcus aureus lipoproteins on immunity of M1 mouse bone marrow-derived macrophages, and provide the theoretical reference for the study of Staphylococcus aureus pathogenicity.In vitro, M1 mouse bone marrow-derived macrophages was infected by WT SA113 and SA113 lgt::ermB strains (SA113Δlgt) of Staphylococcus aureus.The mice were divided into three groups:Blank control group, WT SA113 infection group (MOI:3:1), and SA113Δlgt infection group (MOI:3:1).The tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), chemokines (RANTES) and interleukin-10 (IL-10) in M1 mouse bone marrow-derived macrophages was detected by ELISA method, the expression of Toll-like receptor 2 (TLR2), Toll-like receptor 2(TLR4) and recombinant NLR family, Pyrin domain containing protein 3 (NLRP3) genes were detected by Real-time quantitative PCR, and the effect of lipoproteins on phagocytosis of Staphylococcus aureus by M1 mouse bone marrow-derived macrophages was detected by immunofluorescence.The results showed that compared with control group, both WT SA113 and SA113Δlgt infection could significantly upregulate the secretion of TNF-α, RANTES and IL-10, and WT SA113 infection could significantly increase the expression of TLR2 and NLRP3 genes in M1 mouse bone marrow-derived macrophages polarization (P<0.05), while the expression of TLR4 gene was significantly decreased (P<0.05).Compared with WT SA113 infected group, the secretion of TNF-α, IL-1β, RANTES and IL-10, and the expression of TLR2 (expect for 12 h) and NLRP3 gene in SA113Δlgt infected group were significantly decreased (P<0.05).The immunofluorescence results showed that the phagocytosis effect of M1 macrophage on SA113Δlgt strain was significantly lower than that of WT SA113 strain (P<0.05).In conclusion, the lipoprotein of Staphylococcus aureus induced the production and release of cytokines TNF-α, IL-1β, RANTES and IL-10 mainly through activation of TLR2 and NLRP3 receptors in M1 mouse bone marrow-derived macrophages.

Key words: Staphylococcus aureus; lipoprotein; bone marrow-derived macrophages; cytokines

CLC Number:

S852.61⁺1

ZHANG Jing, QIAN Yinghong, ZHANG Kai, WU Jindi, LIU Bo, MAO Wei, CAO Jinshan. Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3079-3086.

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Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages

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