辣蓼黄酮乙酸乙酯部分对PRV体外诱导RAW264.7细胞分泌炎性因子的影响

doi:10.16431/j.cnki.1671-7236.2021.03.038

Abstract

Abstract: The present study investigated the effect of FEA on inflammatory cytokines secretion in PRV induced RAW264.7 cells.Briefly,CCK-8 method was used to detect the activity of cells in vitro,in order to screen the safe concentrations of FEA on RAW264.7 cells.Then,the groups were assigned as blank control group,PRV positive group,rutin group and five FEA groups.The RAW264.7 cells(except for blank control group)were incubated with PRV for 1.5 h,then cultured with different concentrations of rutin or FEA for 4,8,12 and 24 h,respectively.CCK-8 method was used to detect the activity of PRV induced cells for screen three concentrations of FEA (high,medium and low doses)and then ELISA method were used to detect the secretion levels of TNF-α,IL-1β,IL-6,IL-10,MCP-1 and IFN-γ,in order to evaluate the optimal time and concentration of FEA for anti-inflammatory effect.The results showed as follows:①The safe concentration of FEA was ranged from 12.5 to 200 μg/mL.②In vitro,25-100 μg/mL of FEA could significantly improve the proliferation activity of PRV-induced RAW264.7 cell.③The levels of inflammatory cytokines were increased in PRV induced RAW264.7 cells,treatment of FEA for 8-12 h markedly decreased the levels of TNF-α,IL-1β,IL-6 and MCP-1 (P<0.05),increased the level of IFN-γ when compared with PRV group (P<0.05),and the IL-10 level was also regulated by FEA to a certain extent.Moreover,the anti-inflammatory effect was much better post FEA treatment for 8 h.In conlusion,FEA could regulate the secretion levels of inflammatory cytokines in PRV-induced RAW264.7 cell and exert anti-inflammatory effects in vitro.The results provided reference for further research on the molecular mechanism for antiviral drug.

Key words: ethyl acetate of flavonoids from Polygonum hydropiper L.(FEA); Pseudorabies virus; inflammatory cytokines

CLC Number:

S859.7

REN Chunzhi, LU Caixia, WEI Yingyi, YU Meiling, HU Tingjun. Effect of Ethyl Acetate Fraction of Flavonoids from Polygonum hydropiper L. on Cytokines Secretion in RAW264.7 Cells Induced by Pseudorabies Virus[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1132-1140.

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Effect of Ethyl Acetate Fraction of Flavonoids from Polygonum hydropiper L. on Cytokines Secretion in RAW264.7 Cells Induced by Pseudorabies Virus

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