他莫昔芬对猪伪狂犬病病毒体外增殖的影响

doi:10.16431/j.cnki.1671-7236.2022.11.042

Abstract

Abstract: 【Objective】 The purpose of this study was to study the antiviral effect of Tamoxifen on the infection of porcine Pseudorabies virus (PRV) in PK15 cell model.【Method】 Taking PK15 cells as the model, the effect of Tamoxifen on cell viability was detected by CCK-8 cell counting method.The effects of Tamoxifen on cell cycle and apoptosis were detected by ANNEXIN V-FITC/PI apoptosis kit.CytoFLEX flow cytometry and fluorescence microscope were used to detect the difference of virus proliferation in Tamoxifen treated cells infected with PRV-GFP.The changes of PRV gB gene mRNA and protein expression in Tamoxifen treated cells infected with PRV-QXX were detected by Real-time quantitative PCR and Western blotting, respectively.Virus titer assay was used to detect the inhibition of Tamoxifen treated cells infected with PRV-QXX.【Result】 Tamoxifen had no effect on cell viability at the concentration of 6 μmol/L.When the concentration of Tamoxifen was below 6 μmol/L, compared with control group, Tamoxifen treatment group had no significant effect on cell cycle and apoptosis(P>0.05).At the same time point, the proliferation rate of PRV-GFP in PK15 cells in Tamoxifen treatment group was extremely significantly lower than that in control group(P<0.01).Real-time quantitative PCR showed that Tamoxifen extremely significantly inhibited the mRNA expression of PRV gB gene in PK15 cells (P<0.01).The results of Western blotting showed that Tamoxifen significantly or extremely significantly inhibited the expression of PRV gB protein in the treatment groups with different concentrations at the same time point (P<0.05 or P<0.01).The results of virus titer showed that the replication rate of PRX-QXX progeny virus in Tamoxifen treatment group was significantly or extremely significantly lower than that in control group at the same time point (P<0.05 or P<0.01).【Conclusion】 Tamoxifen could significantly inhibit the proliferation of PRV in PK15 cells.

Key words: Tamoxifen; Pseudorabies virus (PRV); PK15 cell; antiviral effect

CLC Number:

S859.7

XING Jiayou, FAN Wenjie, WANG Yumin, LU Xiuxiang, SONG Yue, CHU Beibei, WANG Jiang, WANG Mengdi. Effect of Tamoxifen on the Proliferation of Porcine Pseudorabies Virus in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4503-4512.

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Effect of Tamoxifen on the Proliferation of Porcine Pseudorabies Virus in vitro

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