T-2毒素致猪肾细胞损伤及氧化应激相关机理的研究

doi:10.16431/j.cnki.1671-7236.2021.12.039

Abstract

Abstract: The purpose of this test was to study the toxic effect of T-2 toxin on porcine kidney cells (PK15) and its oxidative stress response.The cells were treated with different concentrations of T-2 toxin.The cytotoxicity of the toxin was evaluated by MTT assay, intracellular lactate dehydrogenase (LDH) release rate and HE staining.The effects of different doses of T-2 toxin on the level of oxidative stress were evaluated by detecting the levels of reactive oxygen species (ROS), malondialdehyde (MDA), total glutathione (GSH) and total glutathione peroxidase (GPx).The expression levels of Nrf2, Keap1, GPx-1, Nqo1 and Hmox1 genes mRNA related to Nrf2-ARE signal pathway were detected, and the effect of T-2 toxin on this signal pathway was analyzed.The results showed that the activity of PK15 cells decreased in a toxin dose-dependent manner (P<0.05), and the release rate of LDH increased in a toxin dose-dependent manner (P<0.05).With the increase of T-2 toxin dose, the cell gap gradually increased, the cell pyknosis and the number of cells decreased.The rate of ROS positive cells increased in a dose-dependent manner, and when T-2 toxin was 100 nmol/L, the rate of ROS positive cells was significantly higher than that in other dose groups (P<0.05).The intracellular MDA content and GPx activity increased in a toxin dose-dependent manner (P<0.05), while the GSH content decreased in a toxin dose-dependent manner (P<0.05).20, 50 and 100 nmol/L T-2 toxin could significantly up regulate the relative expression of Keap1, GPx-1 and Nqo1 genes mRNA (P<0.05), and the relative expression of 50 nmol/L was the highest.T-2 toxin caused toxic effects on PK15 cells and induced oxidative damage, and the oxidative stress process was regulated by Nrf2-AER signal pathway.

Key words: T-2 toxin; PK15 cells; toxicity; oxidative stress; Nrf2/ARE pathway

CLC Number:

S859.87

XIONG Kuankuan, WU Yingxin, YI Siliang, YANG Lingchen. Mechanism of T-2 Toxin Induced Injury and Oxidative Stress in PK15 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4710-4717.

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Mechanism of T-2 Toxin Induced Injury and Oxidative Stress in PK15 Cells

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