蝇蛆抗氧化肽对IPEC-J2细胞氧化应激损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2023.05.011

Abstract

Abstract: 【Objective】 Taking the antioxidant peptide of fly maggot (FTP) as the research object,the protective effects of FTP on DPPH and ABTS⁺· free radicals scavenging ability and oxidative stress injury of IPEC-J2 cells were investigated using in vitro chemical and cell tests.【Method】 By measuring different concentrations of FTP (500,1 000,2 000,3 000 and 4 000 μg/mL) scavenging rate of DPPH,ABTS⁺· free radicals to detect the antioxidant capacity of FTP in vitro. IPEC-J2 cells were cultured with different concentrations of FTP (0,100,250,500,1 000 and 2 000 μg/mL),and the cell survival rate of each group was measured by MTT method after 24 h of incubation.The appropriate hydrogen peroxide H₂O₂ concentration was selected by MTT method to construct oxidative stress model.According to the constructed model of oxidative stress and the appropriate concentration gradient for FTP,IPEC-J2 cells were randomly divided into blank,model,and test groups (100,250,500 and 1 000 μg/mL),and the levels of cell viability,reactive oxygen species (ROS),and intracellular SOD,CAT and MDA were measured in each group.【Result】 When the concentration of FTP was 4 000 μg/mL,the scavenging rates of DPPH and ABTS⁺· radicals reached 54.82% and 81.90%,respectively.Different concentrations of FTP had no inhibitory effect on the growth of IPEC-J2 cells,and the cell survival rate was higher than 100%,showing a concentration gradient growth,and the cell survival rate of the FTP concentration of 2 000 μg/mL was 1.22 times higher than that of blank group.The suitable concentration for H₂O₂ to construct the cellular oxidative stress model was 1 000 μmol/L.Compared with blank group,the cell survival rate of model group was extremely significantly decreased,the levels of ROS and MDA were extremely significantly increased,and the activities of SOD and CAT were extremely significantly decreased (P＜0.01).Compared with model group,the cell survival rate of 4 test groups was extremely significantly increased (P＜0.01),and the cell survival rate of 500 μg/mL FTP group was 2.37 times that of model group.The levels of ROS and MDA in 500 and 1 000 μg/mL FTP groups were significantly decreased (P＜0.05),and the activities of SOD and CAT were extremely significantly or significantly increased(P＜0.01 or P＜0.05),both in a dose-effect relationship.【Conclusion】 FTP had a significant protective effect on oxidative stress injury of IPEC-J2 cells in a dose-dependent manner.

Key words: maggot antioxidant peptides; hydrogen peroxide (H₂O₂); IPEC-J2 cells; oxidative stress

CLC Number:

S811.3

LI Yongqiang, BAO Ruiying, WANG Qin, QIU Pingfei, LI Xiaochun, SHI Huiyu, ZHANG Haiwen, WANG Xuemei. Protective Effects of Antioxidant Peptides from Maggot on Oxidative Stress Injury of IPEC-J2 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1836-1844.

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Protective Effects of Antioxidant Peptides from Maggot on Oxidative Stress Injury of IPEC-J2 Cells

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