茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响

doi:10.16431/j.cnki.1671-7236.2023.05.010

Abstract

Abstract: 【Objective】 This study was aimed to explore the effect of Agrocybe aegerita polysaccharid (AAP) on immunomodulatory of splenic lymphocytes in mice,in order to provide a basis for elucidating the action mechanism and developing the clinical application of AAP.【Method】 The splenic lymphocytes in mice were taken as the subjects,the MTT method was used to determine the safe concentration of AAP on lymphocytes,and the safe concentrations were selected for subsequent experiments.MTT assay was also used to detect the effect of different concentrations of AAP on the proliferation of splenic lymphocytes in mice.Three control groups of cell,lipopolysaccharide (LPS) and plant hemagglutinin (PHA) were also set at the same time to screen out AAP with the stronger proliferation ability on splenic lymphocytes in mice,flow cytometry was used to detect the effect of AAP on the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ of T lymphocyte subtypes.ELISA method was used to determine the effect of AAP on the contents of interleukin-2 (IL-2),IL-4,tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) in the supernatant of lymphocytes.【Result】 AAP concentration lower than 1 000 μg/mL had not any toxic effect on splenic lymphocytes in mice.Compared with control group,different doses of AAP could extremely significantly cooperate with LPS and PHA to stimulate lymphocyte proliferation (P＜0.01),125 and 250 μg/mL AAP could extremely significantly increase the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ (P＜0.01),and significantly or extremely significantly increase the contents of IL-2,IL-4,TNF-α and IFN-γ secreted by lymphocytes (P＜0.05 or P＜0.01).【Conclusion】 AAP could cooperate with LPS and PHA to stimulate lymphocyte proliferation in spleen of mice,increase the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ on the surface of lymphocytes and the ability of lymphocytes to release cytokines,thus improving the immune function of the body.

Key words: Agrocybe aegerita polysaccharid (AAP); mice; lymphocytes; immunoregulation

CLC Number:

S646.1

LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.

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Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice

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