辣木叶总黄酮对小鼠溃疡性结肠炎防治作用的研究

doi:10.16431/j.cnki.1671-7236.2022.10.037

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the preventive and therapeutic effects of Moringa oleifera leaf flavonoids (MOLF) on ulcerative colitis (UC) in mice induced by dextran sodium sulfate (DSS).【Method】 50 BALB/c mice were randomly divided into 5 groups:control group,DSS group (model group),MOLF-L(25 mg/kg),MOLF-M(50 mg/kg) and MOLF-H(100 mg/kg) groups.Each group contained 10 mice.Mice were induced UC by drinking 4% DSS, and MOLF-L,MOLF-M and MOLF-H groups were given the corresponding doses of MOLF 0.2 mL by gavaged,the control and DSS groups were gavaged with an equal volume of normal saline once a day for 7 d.The disease activity index (DAI) of each group was recorded daily. After 7 d of treatment,the blood was collected from the transorbital venous plexus,and the serum was separated from the blood samples for the determination of IL-1β,IL-10,TNF-α,HMGB1 and LPS.Mice were sacrificed and their colon tissues were harvested for HE staining to observe tissue morphology,and PAS staining to observe goblet cell numbers in tissues. The mRNA expression levels of IL-1 β,IL-10,TNF-α and HMGB1 in colonic tissue were respectively detected by Real-time quantitative PCR,the expressions of zonula occludens-1 (ZO-1) and Occludin were detected by immunofluorescence assay,and the expression levels of apoptosis related proteins cleaved-caspase 3,Bcl-2 and Bax were analyzed by Western blotting.【Result】 UC was successfully established in mice.Compared with the control group,the levels of LPS and inflammatory factors,the expressions of cleaved-caspase 3 and Bax in the DSS group were extremely significantly different (P＜0.01).Different doses of MOLF could improve the inflammation in UC mice,especially in the MOLF-H group:Compared with the DSS group, the DAI score and LPS content were extremely significantly reduced(P＜0.01), the mRNA expression of pro-inflammatory factors IL-1β,TNF-α and HMGB1 in serum and colon tissues were extremely significantly reduced (P＜0.01),the expression of anti-inflammatory factor IL-10 was extremely significantly increased(P＜0.01),besides,cleaved-caspase 3 and Bax were up-regulated and Bcl-2 expression level was down-regulated extremely significantly(P＜0.01).In addition,MOLF could improve colonic mucosal edema and inflammatory cell infiltration,protect the number and morphology of goblet cells,and increase the expression of ZO-1 and Occludin proteins,with a dose-effect relationship between MOLF.【Conclusion】 MOLF could protect against UC by regulating the levels of inflammatory factors,ameliorating DSS induced impairment of ZO-1 and Occludin expression and abnormal expression of apoptosis related proteins in the colon tissues of mice.

Key words: Moringa oleifera leaf flavonoids; mice; ulcerative colitis; inflammatory factor; intestinal mucosal barrier

CLC Number:

S859.7

PENG Weilong, HOSAMELDEEN Mohamed Husien, BO Ruonan, LIU Mingjiang, YANG Haifeng, LI Jingui. Preventive and Therapeutic Effects of Moringa oleifera Leaf Flavonoids on Ulcerative Colitis in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4042-4051.

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Preventive and Therapeutic Effects of Moringa oleifera Leaf Flavonoids on Ulcerative Colitis in Mice

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