添加罗格列酮对小鼠卵母细胞氧化还原稳态的影响

doi:10.16431/j.cnki.1671-7236.2023.05.009

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effects of rosiglitazone (RSG) on the oocyte maturation in vitro and subsequent development potential of embryos in mice,in order to provide reference for the optimization of in vitro maturation (IVM) culture system.【Method】 The optimum concentration of RSG was determined by adding 0,10,20,50 and 100 μmol/L RSG to IVM medium,and calculating the first polar body (PBⅠ) rate. According to the screening results of the RSG optimal concentration,they were divided into control and rosiglitazone groups.Reactive oxygen species (ROS),glutathione (GSH),cathepsin B (CB),mitochondrial membrane potential (MMP),total cell number (Hoechst 33342) and apoptosis ratio (TUNNEL) in oocytes or blastocysts were detected by immunofluorescence staining.The transcription levels of antioxidant genes (Sod-2,Gpx-3 and Cat) and apoptosis-related genes (Caspase-3,Bcl-2 and Bcl-xl) in blastocysts were detected by Real-time quantitative PCR.【Result】 Compared with control group,20 μmol/L RSG supplementation could significantly increase the PBⅠ rate,cleavage rate and blastocyst rate of oocytes in mice (P＜0.05);The intracellular ROS and CB levels were significantly decreased,the intracellular GSH and mitochondrial membrane potential levels were significantly increased (P＜0.05);The total cell number in blastocysts was significantly increased,and the apoptosis rate in blastocysts was significantly decreased (P＜0.05).The transcription levels of antioxidant genes (Sod-2, Gpx-3 and Cat) and anti-apoptotic genes (Bcl-2 and Bcl-xl) were significantly increased, and the transcription level of apoptotic gene Caspase-3 was significantly decreased (P＜0.05).【Conclusion】 The addition of RSG to IVM medium was beneficial to intracellular redox homeostasis of oocytes,which could improve the mitochondrial function,increase the developmental ability of oocytes after IVF,and decrease the intracellular CB level and apoptosis of cells in blastocysts.

Key words: rosiglitazone (RSG); mice; oocyte; in vitro maturation (IVM); in vitro fertilization (IVF)

CLC Number:

S852.1

ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.

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Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice

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