肌抑素对PK15细胞MMP-2/7/9表达的影响

doi:10.16431/j.cnki.1671-7236.2020.02.004

Abstract

Abstract: The study was aimed to reveal the regulation relationship between myostation (MSTN) and matrix metalloproteinase (MMPs),and explore the effects of MSTN on the expression of MMP-2/7/9 in PK15 cells.The backfat tissues of MSTN single allele knockout pig was analyzed by transcriptome sequencing.MSTN knockout PK15 cells line of MSTN single allele knockout PK3108 cells and MSTN double allele knockout L18 cells were prepared,the mRNA and protein expression of MSTN and MMP-2/7/9 in PK15,PK3108 and L18 cells were performed by Real-time PCR and Western blotting,respectively.The results showed that compared with the wild type pigs,the levels of transcription factor C/EBPδ and MMP-2/7 mRNA were extremely significantly reduced (P < 0.01),and the main components of extracellular matrix laminin (LN) and fibronectin (FN) were extremely significantly increased in MSTN single allele knockout pigs (P < 0.01).The revived PK3108 and L18 cells showed green fluorescence.Real-time PCR results showed that the mRNA expression of MSTN and MMP2/7/9 in PK3108 and L18 cells were extremely significantly reduced than that in PK15 cells (P < 0.01).Western blotting results indicated that the protein expression of MSTN and MMP2/7/9 in PK3108 and L18 cells were significantly reduced than that in PK15 cells.The results revealed that the expression of MMP-2/7/9 were significantly decreased by MSTN deletion in PK15 cells,and the expression trend of MMP-2/7/9 protein were consistent with the trend of MSTN protein expression.

Key words: pig; MSTN; MMP-2/7/9; PK15 cell

CLC Number:

S814.8

ZHU Zhe, ZHANG Jianying, HUA Zaidong, REN Hongyan, XIAO Hongwei, ZHANG Liping, ZHENG Xinmin, BI Yanzhen, LU Shengsheng. A Effects of Myostatin on the Expression of MMP-2/7/9 in PK15 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 345-351.

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A Effects of Myostatin on the Expression of MMP-2/7/9 in PK15 Cells

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