Abstract: 【Objective】 This study was aimed to explore the anti-inflammatory effect of 'Ruyan Ning’ water extract,and provide theoretical basis for its clinical application and further research.【Method】 The anti-inflammatory effects of xylene-induced auricle swelling,acetic acid-induced increased peritoneal capillary permeability,carrageenan-induced foot swelling,cotton ball-induced granulation swelling,and serum cytokines contents in mice were studied.60 Kunming mice,half male and half female,were randomly divided into 5 groups,namely blank control group (normal saline group),positive control group (aspirin group),and 'Ruyan Ning’ water extract low (4 mg/g),medium (8 mg/g) and high (16 mg/g) dose groups, 12 mice in each group.Mice in each group were intragastrically administered at a fixed time every day,once a day,for 10 consecutive days.Follow-up according to the specific operation of different experiments,after the experiment,the auricle weight,D590 nm value of peritoneal washing fluid,foot weight,cotton ball granuloma weight and serum contents of tumor necrosis factor-α (TNF-α) and interleukin-1β(IL-1β) were measured.【Result】 The results of the xylene-induced auricle swelling test showed that compared with blank control group,the drug treatment group could extremely significantly reduce the auricle swelling caused by xylene (P<0.01).Compared with positive control group,there was no significant difference in auricle weight in the high-dose group (P>0.05).The results of the acetic acid-induced peritoneal capillary permeability test showed that,compared with the blank control group,the drug treatment group had a very significant inhibitory effect on the increase of acetic acid-induced peritoneal capillary permeability (P<0.01).Compared with positive control group,the capillary permeability of each dose group of 'Ruyan Ning’ water extract was significantly or extremely significantly increased (P<0.05;P<0.01).The results of the carrageenan-induced foot swelling test showed that,compared with the blank control group,the drug treatment group had extremely significant inhibitory effect on the foot swelling of mice (P<0.01).Compared with the positive control group,there were extremely significant differences in the degree of foot swelling in each dose group of the 'Ruyan Ning’ water extract (P<0.01).The results of the cotton ball-induced granulation test showed that,compared with the blank control group,the drug treatment group had a very significant inhibitory effect on the inhibition of cotton ball granulation in mice (P<0.01).Compared with the positive control group,the difference between the low and medium dose groups of the 'Ruyan Ning’ water extract was extremely significant or significant (P<0.01;P<0.05),and the high dose group of 'Ruyan Ning’ had the same effect as the positive control group (P>0.05).The results of the detection of serum cytokines in mice showed that compared with the blank control group,except for the low dose 'Ruyan Ning’ water extract group,the contents of TNF-α and IL-1β in serum in the drug treatment group were reduced to varying degrees,'Ruyan Ning’ water extract low dose group had no significant difference in the content of TNF-α in serum (P>0.05),but the content of IL-1β in serum decreased significantly (P<0.05).The content of IL-1β in serum of medium dose group and high dose group were significantly decreased (P<0.01),and the content of TNF-α in serum was significantly decreased in medium dose group (P<0.05),and extremely significantly decreased in high dose group (P<0.01).Compared with the positive control group,there was no significant difference between the two cytokines in serum of the 'Ruyan Ning’ water extract medium and high dose groups (P>0.05),and two cytokines in the serum of the low dose group had extremely significant differences (P<0.01).【Conclusion】 'Ruyan Ning’ water extract had good anti-inflammatory effect on mouse.

Key words: Ruyan Ning'; water extract; cytokine; anti-inflammatory