China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (6): 2262-2270.doi: 10.16431/j.cnki.1671-7236.2021.06.041

• Basic Veterinary Medicine • Previous Articles    

Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract

XIANG Rong1,3, LI Yahui1, GAO Yuan4, WEI Qilin1, SHU Bingyao1, LUO Shengjun1,2   

  1. 1. Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Chinese Traditional Medicine Engineering Technology Research Center of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangdong Qianyan Animal Health Co., Ltd., Guangzhou 510550, China;
    3. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China;
    4. Zhuhai Qixinda Biotechnology Co., Ltd., Zhuhai 519000, China
  • Received:2021-01-05 Online:2021-06-20 Published:2021-06-18

Abstract: To explore the feasibility and safety of Vitex negundo L.var.cannabifolia ( Zucc.)Hand. -Mazz.(V.negundo) as a traditional Chinese veterinary medicine,the acute toxicity test and 30-day feeding test were carried out on V.negundo water extract.In the acute toxicity experiment,20 SPF Kunming mice were randomly divided into drug group and blank control group.The drug group was intragastrically administered at a crude drug concentration of 40 mg/g body weight,and the blank control group was intragastrically administered with an equal volume of normal saline for continuous observation for 7 days.Repeat the test once more.The 30-day feeding test used 80 SPF SD rats,which were randomly divided into four groups,high (40 mg/g body weight),medium (20 mg/g body weight) and low (10 mg/g body weight) dose groups,and blank control group,half male and female in each group.Drug feeding were continuously administed for 30 days.Weighed once a week,blood was collected after weighing on the 30th day to test hematological and blood biochemical indicators,organs were weighed to calculate the organ coefficient,the main organs of high-dose group and the blank control group were fixed and then were made slices to observe the tissue lesions.The results showed that LD50 of V.negundo water extract was >40 000 mg/kg in the acute toxicity test.In 30-day feeding test,the overall condition of the rats was good throughout the test period.The body weight of high-dose group was extremely significantly lower than those in blank control group (P<0.01),there was no significant difference in other groups.The organ coefficients of spleen of male rats in the high-dose group and testis of male rats in middle-dose group were significantly higher than that in blank control group (P<0.05),the organ coefficient of testis of male rats in high-dose group was extremely significantly higher than that in blank control group (P<0.01),there was no significant difference in the organ coefficient between the groups of female rats.The number of monocytes in middle-dose and low-dose groups were extremely significantly and significantly higherthan that of blank control group (P<0.01;P<0.05),respectively,there was no significant difference between other hematological indexes and blood biochemical indexes (P>0.05),and they were all within the normal range.Histopathological examination showed no siginificant changes.It showed that V.negundo water extract had no obvious acute and subacute toxicity under the experimental conditions,and had high safety.V.negundo water extract could be used as a safe Chinese veterinary medicine for further development and utilization.

Key words: Vitex negundo L. var.cannabifolia ( Zucc.) Hand. -Mazz.water extract; acute toxicity; 30-day feeding test; safety

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