牡荆水提物急性毒性与30 d喂养试验研究

doi:10.16431/j.cnki.1671-7236.2021.06.041

Abstract

Abstract: To explore the feasibility and safety of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.)Hand. -Mazz.(V.negundo) as a traditional Chinese veterinary medicine,the acute toxicity test and 30-day feeding test were carried out on V.negundo water extract.In the acute toxicity experiment,20 SPF Kunming mice were randomly divided into drug group and blank control group.The drug group was intragastrically administered at a crude drug concentration of 40 mg/g body weight,and the blank control group was intragastrically administered with an equal volume of normal saline for continuous observation for 7 days.Repeat the test once more.The 30-day feeding test used 80 SPF SD rats,which were randomly divided into four groups,high (40 mg/g body weight),medium (20 mg/g body weight) and low (10 mg/g body weight) dose groups,and blank control group,half male and female in each group.Drug feeding were continuously administed for 30 days.Weighed once a week,blood was collected after weighing on the 30th day to test hematological and blood biochemical indicators,organs were weighed to calculate the organ coefficient,the main organs of high-dose group and the blank control group were fixed and then were made slices to observe the tissue lesions.The results showed that LD₅₀ of V.negundo water extract was >40 000 mg/kg in the acute toxicity test.In 30-day feeding test,the overall condition of the rats was good throughout the test period.The body weight of high-dose group was extremely significantly lower than those in blank control group (P<0.01),there was no significant difference in other groups.The organ coefficients of spleen of male rats in the high-dose group and testis of male rats in middle-dose group were significantly higher than that in blank control group (P<0.05),the organ coefficient of testis of male rats in high-dose group was extremely significantly higher than that in blank control group (P<0.01),there was no significant difference in the organ coefficient between the groups of female rats.The number of monocytes in middle-dose and low-dose groups were extremely significantly and significantly higherthan that of blank control group (P<0.01;P<0.05),respectively,there was no significant difference between other hematological indexes and blood biochemical indexes (P>0.05),and they were all within the normal range.Histopathological examination showed no siginificant changes.It showed that V.negundo water extract had no obvious acute and subacute toxicity under the experimental conditions,and had high safety.V.negundo water extract could be used as a safe Chinese veterinary medicine for further development and utilization.

Key words: Vitex negundo L. var.cannabifolia (Sieb.et Zucc.) Hand. -Mazz.water extract; acute toxicity; 30-day feeding test; safety

CLC Number:

S853.7

XIANG Rong, LI Yahui, GAO Yuan, WEI Qilin, SHU Bingyao, LUO Shengjun. Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2262-2270.

References

[1] LI M M,LI Y T,HUANG Z,et al.A new flavonoid glycoside from leaves of Vitex negundo var. canna-bifolia[J].Chinese Traditional & Herbal Drugs,2015,12:16-19.
[2] 李春正,苏艳芳,靳先军.牡荆属植物化学成分及生物活性研究进展[J].中草药,2005,6:930-938. LI C Z,SU Y F,JIN X J.Advances in studies on chemical constituents from plants of Vitex L.and their bioactivities[J].Chinese Traditional and Herbal Drugs,2005,6:930-938.(in Chinese)
[3] 李华强.牡荆子的化学成分研究以及生药学鉴定[D].上海:上海师范大学,2015. LI H Q.Chemical constituents and pharmacognostical identification of Vitex negundo var. cannabifolia fruits[D].Shanghai:Shanghai Normal University,2015.(in Chinese)
[4] 凌玮玮.牡荆化学成分及其抑菌活性研究[D].合肥:安徽农业大学,2010. LING W W.Constituents and antibacterial activities of Vitex negundo Linn. var. cannabifolia[D].Hefei:Anhui Agricultural University,2010.(in Chinese)
[5] 谭武宾,米长忠,谢庭裕,等.黄荆条果实对糖尿病模型血糖的影响[J].四川中医,2018,36(8):42-44. TAN W B,MI C Z,XIE T Y,et al.Effect of Huang Jingtiao’s fruit on glycemia of diabetic model[J].Journal of Sichuan of Traditional Chinese Medicine,2018,36(8):42-44.(in Chinese)
[6] 尹爱武,黄赛金,罗紫英,等.蔓荆子醇提物抗衰老与抗花生油氧化作用研究[J].中国粮油学报,2016,31(9):91-94. YIN A W,HUANG S J,LUO Z Y,et al.The anti-aging and antioxidant effects of alcohol abstracts from Vitex trifolia L.on peanut oils[J].Journal of the Chinese Cereals and Oils Association,2016,31(9):91-94.(in Chinese)
[7] SICHAEM J,NGUYEN H H,NGUYEN V H,et al.A new labdane-type diterpenoid from the leaves of Vitex negundo L.[J].Natural Product Research,2019,10:1-6.
[8] 赵群,娄志华,钟金栋,等.牡荆子的化学成分及抗氧化活性研究[J].昆明理工大学学报(自然科学版),2016,41(5):92-99. ZHAO Q,LOU Z H,ZHONG J D,et al.Chemical constituents from the seeds of Vitex negundo var.cannabifolia and their antioxidant activities in vitro[J].Journal of Kunming University of Science and Technology (Natural Science),2016,41(5):92-99.(in Chinese)
[9] HUANG Q,LIN C W,HUANG K J,et al.Essential oil in leaves,stems and flowers from Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand. -Mazz[J]. Lishizhen Medicine & Materia Medica Research,2007,4:47-49.
[10] WU Y,YOU C X,TIAN Z F,et al.Chemical composition and insecticidal activities of Vitex negundo L.var. cannabifolia (Sieb.et Zucc.)Hand. -Mazz.essential oil against the cigarette beetle[J].Plant Protection,2016,5:102-107.
[11] TANG Y,CHEN G,ZHANG J,et al.Chemical composition and the regional effect of Vitex negundo Linn.var.cannabifolia (Sieb.et Zucc.) Hand. -Mazz.essential oils and the regional effect on it[J].Journal-Chemical Society of Pakistan,2011,33(5):744-746.
[12] HUANG Q,TIAN Y H,LIN C W,et al.Study on extraction technology of total flavonoids from medicinal plant Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand. -Mazz[J].Medicinal Plant,2011,4:55-56.
[13] 贾少强,陈燕杰,张学武.中草药在猪和家禽饲料中的应用潜力和挑战[J].中国饲料,2019,20:42-46. JIA S Q,CHEN Y J,ZHANG X W.Potential and challenge for application of Chinese herbs in pig and poultry feed[J].China Feed,2019,20:42-46.(in Chinese)
[14] 李琳倩,张云龙,刘日亮,等.中草药添加剂对后备母猪生长性能、发情率、血清指标和肠道微生物的影响[J].动物营养学报,2020,32(6):2586-2600. LI L Q,ZHANG Y L,LIU R L,et al.Effects of compound Chinese herbal medicine on growth performance,estrus rate,serum indices and intestinal microflora of replacement gilts[J].Chinese Journal of Animal Nutrition,2020,32(6):2586-2600.(in Chinese)
[15] 杨杰,沙金丹,高翔,等.黄酮类化合物的免疫调节作用及机制[J].动物营养学报,2017,29(12):4295-4300. YANG J,SHA J D,GAO X,et al.Immune regulation function of flavonoids and its mechanisms[J].Chinese Journal of Animal Nutrition,2017,29(12):4295-4300.(in Chinese)
[16] 崔耀成,买占海,库莱夏·阿力别克,等.中草药复合添加剂的小鼠急性毒性试验[J].中国畜牧兽医,2018,45(12):3579-3586. CUI Y C,MAI Z H,KULAIXIA·A,et al.An experimental of mice acute toxicological test of Chinese herbal medicine compound additive[J].China Animal Husbandry & Veterinary Medicine,2018,45(12):3579-3586.(in Chinese)
[17] 韦芊含,雷伟伟,陈昕,等.饲用黄丝藻粉的急性毒性和亚慢性毒性试验[J].中国畜牧兽医,2020,47(5):1602-1610. WEI Q H,LEI W W,CHEN X,et al.Acute toxicity and subchronic toxicity test of Tribinema powder[J].China Animal Husbandry & Veterinary Medicine,2020,47(5):1602-1610.(in Chinese)
[18] 农业部兽药评审中心.兽药研究技术指导原则汇编[M].北京:化学工业出版社,2012. VETERINARY DRUG EVALUATION CENTER OF THE MINISTRY OF AGRICULTURE.Compilation of Guidelines for Veterinary Drug Research Technology[M].Beijing:Chemical Industry Press,2012.(in Chinese)
[19] 李晓亮,邵旭,牛海军,等.Beagle犬牡荆素急性毒性实验研究[J].临床合理用药杂志,2017,22:56-57. LI X L,SHAO X,NIU H J,et al.Acute toxicity study of Vitexin in Beagle dogs[J].Chinese Journal of Clinical Rational Drug Use,2017,22:56-57.(in Chinese)
[20] 舒柄垚,彭新宇,魏文康,等.牡荆的化学成分及药理作用研究进展[J].动物医学进展,2020,41(5):105-110. SHU B Y,PENG X Y,WEI W K,et al.Progress on chemical constituents and pharmacological effects of Vitex negundo var. cannabifolia[J].Progress in Veterinary Medicine,2020,41(5):105-110.(in Chinese)
[21] 徐尧,何永志,李安平,等.蔓荆子化学成分的研究[J].国际药学研究杂志,2019,46(11):848-854. XU Y,HE Y Z,LI A P,et al.Chemical constituents of Viticis Fructus[J].Journal of International Pharmaceutical Research,2019,46(11):848-854.(in Chinese)
[22] SHU Z H,LI H Q,LI Q,et al.Content determination of vitexdoin in the fruits of four Vitex species by HPLC method[J].Pharmaceutical Care & Research,2015,15(4):277-280.
[23] HUANG M Q,ZHANG Y P,XU S Y,et al.Identification and quantification of phenolic compounds in Vitex negundo L. var. cannabifolia (Siebold et Zucc.) Hand.-Mazz.using liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometers[J].Journal of Pharmaceutical and Biomedical Analysis,2015,108:11-20.
[24] 李美发,陈作栋,梁欢,等.添加复方中草药对育肥猪生长性能、营养物质表观消化率和血液指标的影响[J].中国畜牧兽医,2019,46(6):1636-1643. LI M F,CHEN Z D,LIANG H,et al.Effects of compound Chinese herbal medicine on growth performance,nutrient apparent digestibility and blood parameters in finishing pigs[J].China Animal Husbandry & Veterinary Medicine,2019,46(6):1636-1643.(in Chinese)

Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Yuanyuan, YANG Na, TENG Da, HAO Ya, MA Xuanxuan, MAO Ruoyu, WANG Jianhua. Preparation and Evaluation of Efficacy and Safety of Shengtaicin Post-medicated Bath Solution for Dairy Cow [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1617-1631.
[2]	ZHANG Wen, QIN Zhibiao, CHENG Jing, YU Meiling, WEI Yingyi, LI Jiang, HU Tingjun. Study on Acute and Subchronic Toxicity of Polygonum hydropiper L. Extract [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1532-1544.
[3]	QIAO Chunyu, LIU Jiahe, ZHANG Boxi, HE Yuxi, ZHENG Yuwei, LYU Hongming. Research Progress on Pathogenic Mechanism and Control of Aflatoxin B₁ [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 765-775.
[4]	CUI Yiyan, DENG Dun, TIAN Zhimei, LU Huijie, LIU Zhichang, LI Jiazhou, MA Xianyong, ZHANG Qi, LAN Yonghui. Research Progress on Nutritional Value and Feed Safety of Food Waste [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2820-2829.
[5]	GUO Yuli, LIU Junping, SUN Di, DONG Zhiying, WANG Peng, LIU Chenggong. Study on Toxicity and Safety Evaluation of Qinzhu Antai Powder [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3880-3888.
[6]	WEI Qianhan, LEI Weiwei, CHEN Xin, ZHAO Weiya, ZHANG Meng, XIAO Jinzha, ZHANG Yumei. Acute Toxicity and Subchronic Toxicity Test of Tribonema Powder [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1602-1610.
[7]	YANG Shuai, HU Xiangwei, ZHOU Di, ZHANG Xinxin, YANG Liguo. Study on the Growth Promoting Effect of Crossbred Buffalo Calves Immunized with DNA Vaccine of Co-expression of Somatostatin and Cortistatin [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4041-4050.
[8]	HE Mengru, ZHOU Ni, LYU Xiaohua. Risk Assessment of Bacitracin Resistance as a Medicated Feed Additive [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3749-3756.
[9]	WENG Jingyu, LIANG Liqing, ZHOU Jingkai, LIU Yuting, LI Li. Acute Toxicity and Hypoglycemic Activity of Total Flavonoids from Melastoma dodecandrum Lour. [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 275-281.
[10]	LIU Dingxi, YANG Jiecheng, ZHOU Hongchao, GUO Kangkang. Research Advance on Detection Methods of Antibacterial Residues in Animal Food [J]. , 2019, 46(7): 2183-2192.
[11]	ZENG Fanmei, CHONG Yuqing, JIANG Xunping, LIU Guiqiong. Research Progress on Immuno-castration in Livestock [J]. , 2019, 46(6): 1739-1745.
[12]	JIN Ye, LIANG Pengfei, FANG Mei, ZHANG Jianan, JIA Ning, DENG Kang, HUANG Weikuan. Extraction,Purification,Acute Toxicity and Antioxidant Activity of Total Flavonoids from Seeds of Ammopiptanthus [J]. , 2019, 46(3): 940-948.
[13]	SUN Jichao, DONG Zhen, CHEN Chen, ZHANG Jiyu, ZHOU Xuzheng. Experimental Study on Oral Acute Toxicity of Florfenicol Premix in Wistar Rats [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3785-3791.
[14]	LUO Lirong, WANG Hui, CUI Dongan, WANG Fuhe, WANG Shengyi. Study on Acute and Sub-chronic Toxicity Study of Zixiekang Oral Liquid [J]. , 2018, 45(9): 2582-2590.
[15]	WEN Xuemei, LAI Shengji, HU Tingjun, CUI Yumei, LU Yanrong, ZHANG Xinqin, TANG Tingchong, YANG Shanzhong, HE Jiakang. Safety Study of Sarcandra glabra Sanqing Granules [J]. , 2018, 45(9): 2600-2609.