China Animal Husbandry and Veterinary Medicine ›› 2019, Vol. 46 ›› Issue (12): 3785-3791.doi: 10.16431/j.cnki.1671-7236.2019.12.039

• Basic Veterinary Medicine • Previous Articles     Next Articles

Experimental Study on Oral Acute Toxicity of Florfenicol Premix in Wistar Rats

SUN Jichao1,2, DONG Zhen1,2, CHEN Chen1,2, ZHANG Jiyu1,2, ZHOU Xuzheng1,2   

  1. 1. Key Laboratory of Animal Drug Production in Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
    2. Key Laboratory of New Veterinary Medicine Engineering in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
  • Received:2019-07-16 Published:2019-12-21

Abstract: The purpose of this study was to evaluate the acute oral toxicity of florfenicol premix to Wistar rats.The median lethal dose (LD50) was measured using the modified Up-and-Down Procedure (UDP) revised by OECD.The adverse effects of florfenicol premix on the biological system and main organs of Wistar rats were determined by weight gain,organ coefficient,haematology,clinical chemical examination and histopathological examination.According to the LD50>5 000 mg/kg of florfenicol,the limit test of UDP was selected,and the toxic reaction and death were recorded by using a fixed number (5) of animals with a dose of 2 000 mg/kg for 14 days.LD50 was calculated by AOT425StatPgm program,and the other 3 Wistar rats were given the same dose of normal saline as control.The results showed that none of the five rats died,LD50>2 000 mg/kg.During the test,there were no signs of toxic reaction in the treatment group.Compared with the control group,the haematological parameters in the treatment group had no significant change,but in the clinical chemical examination,the level of ALT in the treatment group was significantly higher than that in the control group (P<0.05),suggesting the toxic damage of the pharmaceutical preparation to the liver.The results of histopathological examination showed that the treatment group had no toxic damage to the main organs,such as heart,liver,spleen,lung,kidney and duodenum,and the toxic target organs could not be determined for the time being.The results showed that the use of FF premix in the safe dose range was safe and reliable,and more toxicity information still needed to be determined by long-term toxicity test.

Key words: florfenicol premix; oral acute toxicity; up-and-down procedure; median lethal dose; haematology; clinical chemistry; histopathology; target organ

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