绵羊感染伪狂犬病的病理学观察与病毒分离鉴定

doi:10.16431/j.cnki.1671-7236.2015.02.020

Abstract

Abstract: To diagnose a suspected sheep pseudorabies case,the tissue samples of sheep with clinical symptom of sheep pseudorabies from a farm in Shanghai suburb were collected for pathological observation,isolation and identification of the virus.The histopathological observation of sheep showed extensive neuronal degeneration and necrosis occurred in the brain tissue accompanied with neurotropic phenomenon and gliosis.BHK-21 cells inoculated by tissue samples showed lesions.Pseudorabies virus (PRV) in BHK-21 cells was confirmed by specific fluorescence of indirect immunofluorescence assay and fluorescence PCR.Epidemiological investigations showed sheep pseudorabies was possibly caused by swine pseudorabies virus.Sheep pseudorabies was controlled in time by inoculating swine pseudorabies attenuated vaccine.

Key words: sheep pseudorabies; histopathology; cell culture; fluorescence PCR

CLC Number:

S855.3

QI Xin-yong, LIU Jian, ZHOU Jin-ping, JU Hou-bin, GE Fei-fei, ZHANG Wei-yi, XU Feng, SHEN Li-ping, NING Kun, WANG Jian, LIU Pei-hong. Pathological Observation of Sheep Infected Pseudorabies Virus, and Isolation and Identification of the Virus[J]. , 2015, 42(2): 365-369.

[1]	HUANG Xiaohang, LI Shiyi, LI Yongxia, LI Mengrui, HE Jinyu, ZHANG Long, LIU Daoquan, JIANG Heji, HUANG Zhijian, YIN Guangwen. Clinical Case Study of Toad Infected with Sphaerirostris lanceoides [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2423-2430.
[2]	XIE Zimin, ZENG Fancong, FU Yuming, JIANG Hanyu, YANG Huihu, KE Junhong, LUO Rui, LIANG Zhaoping, ZHANG Xuelian, HUANG Shujian. Study on Pathogenicity of 4 Strain of Subgroup Ⅰ Fowl Adenovirus Isolates to SPF Chicks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1032-1040.
[3]	XIAO Lu, YU Jifeng, LIN Yi, ZHOU Long, GUO Zhiqiang, XIE Jing, YUE Jianguo, YE Yonggang, CAO Ye, LI Xingyu, PAN Meng, YE Jianqiang, LI Min, KANG Runmin. First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 348-355.
[4]	WANG Shiyu, LI Botong, LIU Jiawei, NI Yaodi, LIU Mingchao. Improvement of Culture Method of Primary Bovine Endometrial Epithelial Cells in Vitro [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 958-964.
[5]	LI Wen, HAN Yuping, ZHAO Xiangyuan, FAN Bingfeng, LI Xiaoxia, DIAO Yunfei, YANG Yifeng, XU Baozeng. American Mink Ear Fibroblasts Transfected by Adenovirus Vector [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 37-43.
[6]	GUO Yuqi, SUN Dongmei, PENG Zhaoyu, JIANG Xunping, HU Xueying, LIU Yun, LIU Guiqiong. Isolation and Primary Culture of Epithelial Cells of Sperm Storage Tubules in Hens [J]. , 2019, 46(5): 1323-1329.
[7]	SUN Jichao, DONG Zhen, CHEN Chen, ZHANG Jiyu, ZHOU Xuzheng. Experimental Study on Oral Acute Toxicity of Florfenicol Premix in Wistar Rats [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3785-3791.
[8]	CHANG Hongjian, REN Xiaoli, ZHANG Pei, ZHAO Bingbing, XIAO Min, LI Yongqi, WANG Sen, WU Yuhong, LIU Na, LIU Yun. Epidemiological Investigation and Histopathological Diagnosis of Common Tumors in Dogs [J]. , 2018, 45(5): 1417-1425.
[9]	LIU Zhi-ke, YANG Ning-ning, XU Jin-feng, XU Ming-guo, JING Ming-long, WU Wen-xing, CAO Xu-dong, REN Yan, SHI Feng, CHEN Chuang-fu. Isolation, Identification and Analysis of Drug Resistance of Salmonella Pullorum [J]. , 2017, 44(9): 2792-2800.
[10]	WANG Jing-yuan, WU Qiao-xing, SHE Rui-ping, LI Heng, SHI Rui-han, YANG Jin-ling, HAO Wen-zhuo, AN Jun-qing, YUAN Xue-rui, HU Feng-jiao. Histopathologic Diagnosis and Analysis of 44 Cases of Pet Dog Tumors [J]. , 2017, 44(3): 935-940.
[11]	LI Xiao-ling, YU Guang-hui, SUN Jin-hai. Establishment and Identification of a Fetal Fibroblast Cell Line with Double Transgenic (pGH/IGF-Ⅰ) Porcine [J]. , 2016, 43(8): 2053-2059.
[12]	LUO Hai-yan, DOU Bing-ran, JIANG Kai-wei, HONG Hou-sheng. Research Progress on Technology of Fed-batch in Animal Cell Culture [J]. , 2016, 43(1): 176-181.
[13]	ZHU Xue-min, WEI Lan, ZHANG Zi-qiang, DENG Wen, SUN Yu, ZHAO Qian-ning, WANG Meng-fan. Isolation,Culture and Multiple Differentiation Induction of Nanyang Bovine Bone Marrow Mesenchymal Stem Cells [J]. , 2015, 42(9): 2377-2383.
[14]	CHEN Ning-bo, ZHANG Chong-yu, JIANG Shu-zhen, Huang Li-bo, CHEN Xiang-xing, SUN Jian-li, YANG Wei-ren. Effects of Fusarium Toxins on Serum Enzymes Activity,Liver Antioxidant Function and Histopathology of Weaned Gilts [J]. , 2015, 42(9): 2384-2390.
[15]	WANG Qi-hui, ZHU Li-li, SHI Yong-qiang, ZHU Juan, QU Wei-jie. Study on Infection of Staphylococcus aureus Small Colony Variants on Mammary Epithelial Cells in Vitro [J]. , 2015, 42(8): 2169-2175.

Pathological Observation of Sheep Infected Pseudorabies Virus, and Isolation and Identification of the Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments