转双基因(pGH/IGF-Ⅰ)猪胎儿成纤维细胞系的建立及鉴定

doi:10.16431/j.cnki.1671-7236.2016.08.018

Abstract

Abstract:

This study was aimed to establish a fetal fibroblast cell line of double transgenic (pGH/IGF-Ⅰ) pigs,and reserve fibroblast cells of double transgenic pigs for the follow-up study.A method of trypsin digestion was adopted to isolate and culture body tissues from pregnant porcine,and porcine fetal fibroblasts were isolated successfully through primary culture,subculturing and cryopreservation.The morphological observation,determination of viability before cryopreservation and after recovery,dynamic growth analysis,vimentin immunohistochemistry and microbial contamination detection were all done to study the biological characteristics of the cell line.The results showed that the fibroblasts were cultured and isolated successfully by trypsin digestion and differential centrifugation.The cell viability before cryopreservation and after recovery were 94.3% and 91.2%,respectively.The growth curve was sigmoidal,and experienced the incubation period,exponential growth period and platform three stages.The vimentin immunohistochemistry was positive,the microbial contamination detection were all negative.The results indicated that a fibroblast cell line of double transgenic porcine was successfully established.

Key words: double transgenic porcine; fibroblast cell line; cell culture; biological characteristics

CLC Number:

Q343.6

LI Xiao-ling, YU Guang-hui, SUN Jin-hai. Establishment and Identification of a Fetal Fibroblast Cell Line with Double Transgenic (pGH/IGF-Ⅰ) Porcine[J]. , 2016, 43(8): 2053-2059.

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Establishment and Identification of a Fetal Fibroblast Cell Line with Double Transgenic (pGH/IGF-Ⅰ) Porcine

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