1株牦牛瘤胃源无色杆菌的鉴定及生物学特性评价

doi:10.16431/j.cnki.1671-7236.2022.12.038

Abstract

Abstract: 【Objective】 In order to develop a new type of animal-derived probiotics, a yak rumen strain was identified and its biological characteristics were analyzed.【Method】 The tested strains were identified by microscope and colony observation, and its biochemical characteristics was identified by VITEK2 microbiological automatic identification instrument.The 16S rDNA was amplified by PCR, and similarity comparison and genetic evolution tree construction were carried out.The growth performance, acid producing ability, self-coagulation, hydrophobicity, hemolysis and drug resistance, acid and bile salt resistance and gastrointestinal environmental adaptability were studied.【Result】 After morphological, molecular identification and 16S rDNA amplification, the test strain was identified as Achromobacter and named as Achromobacter YKS2.The strain entered logarithmic phase at 2 h, and continued to grow to 20 h, and began to plateau and slowly decline.The pH did not decrease after 48 h fermentation, indicating that the strain did not have acid production ability.The self-cohesion was 28.7%, the surface hydrophobicity was 77.1% in chloroform, and the other solvents were more than 60.0%, with an average of more than 60%, indicating that the bacteria had certain adhesion ability.There was no hemolytic ring after 48 h incubation on blood agar plate.The sensitivity to 14 antibiotics was tested by K-B method, and it was found that it was sensitive to 12 common antibiotics.The strain could not survive in the medium with pH 2.0, and the survival rate was 61.4% in the medium with pH 4.0.It had high tolerance to bile salt medium containing 0.1% and 0.3%.The survival rate of bacteria cultured in artificial gastric juice for 3 h was 78.18%.After treatment in artificial intestinal juice for 4 and 8 h, the number of colonies increased significantly compared with the control group (P<0.05), indicating that YKS2 strain could tolerate artificial intestinal juice to a certain extent.【Conclusion】 YKS2 strain derived from yak rumen was a subspecies of Achromobacillus of Enterobacteriaceae with no hemolysis, acid production and strong acid resistance.It was a non-drug resistant strain with good proliferation performance, and had the properties of bile salt resistance, gastric fluid resistance, self-condensation and surface hydrophobicity.It could be used as a candidate probiotic strain for further evaluation and study.

Key words: yak; rumen; Achromobacter; biological characteristics

CLC Number:

S852.69

LI Dapeng, CAO Chunhua, FU Pengcheng, ZHANG Jingyan, ZHANG Kang, WANG Lei, ZHANG Kai, LIU Qin, BAO Yongzhan, LI Jianxi. Identification and Biological Characteristics Evaluation of an Achromobacter Strain Isolated from Rumen of Yak[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4909-4919.

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Identification and Biological Characteristics Evaluation of an Achromobacter Strain Isolated from Rumen of Yak

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