中国首例兔出血症病毒2型(RHDV2/b/GI.2)的鉴定及病理学观察

doi:10.16431/j.cnki.1671-7236.2021.01.038

Abstract

Abstract: The purpose of this study was to identify the suspected etiology of Rabbit hemorrhagic virus type 2 (RHDV2) infection in a rabbit farm in Jintang,Sichuan province,and to analyze the pathological histological changes of the infected rabbits.Hemagglutination test (HA) and RT-PCR were used to detect and identify the pathogens of the rabbit,and then pathological sections were taken from the diseased tissue to observe and analyze the histopathological changes of each tissue.At the same time,liver suspension was used to infect young rabbits,and the virulence of this strain was analyzed.The results of HA showed that liver samples collected from diseased and dead rabbits could agglutinate human "O" red blood cells.RT-PCR and sequencing results showed that RHDV2 specific bands were amplified from the samples with multiple pairs of primers.The histopathology microscopic examination revealed that the rabbit displayed systemic hemorrhagic and internal swelling with infiltration of large lymphocytes and neutrophils,and the hemorrhagic in liver,lung,and tracheal mucosa were the most serious.The animal test results showed that this strain was highly virulent,and the liver suspension containing RHDV2 was lethal to young rabbits and caused death in 24 h.In this study,clinical diagnosis,nucleic acid testing and sequencing confirmed that the outbreak was caused by RHDV2 infection.Animal tests and histopathological observation showed that this strain was virulent,which could cause serious bleeding organs,resulting in acute death of the sick rabbit.The emergence of RHDV2 indicated that the cross-border spread of virus was not optimistic,and it should be seriously monitored.

Key words: rabbit hemorrhagic disease; Rabbit hemorrhagic disease virus type 2(RHDV2/b/GI.2); histopathology; virulence

CLC Number:

S855.3

XIAO Lu, YU Jifeng, LIN Yi, ZHOU Long, GUO Zhiqiang, XIE Jing, YUE Jianguo, YE Yonggang, CAO Ye, LI Xingyu, PAN Meng, YE Jianqiang, LI Min, KANG Runmin. First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 348-355.

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First Detection of a Type 2 Rabbit Hemorrhagic Disease Virus (RHDV2/b/GI.2) in China and Its Pathological Observation

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