福建省漳州市罗非鱼无乳链球菌分离鉴定及毒力基因和耐药性研究

doi:10.16431/j.cnki.1671-7236.2022.09.039

Abstract

Abstract: 【Objective】 The purpose of this study was to clarify the serotype, virulence gene carrier and drug resistance characteristics of clinical isolates of Streptococcus agalactiae (S.agalactiae) from tilapia in Zhangzhou, Fujian, and to provide new data for the study of S.agalactiae from fish.【Method】 Samples of diseased fish suspected of S.agalactiae infection were collected from some tilapia farms in Zhangzhou city, Fujian province.The isolates were identified by PCR identification and sequencing alignment through 16S rDNA-specific fragment primers and 16S rDNA universal primers.The serotypes of the isolates and the distribution of four major virulence genes (cylE, sodA, gapC and scpB genes) were detected by PCR.The susceptibility tests of 29 kinds of antibiotics in 11 categories was tested by disk diffusion method, and the multi drug resistance was analyzed.【Result】 The isolated colonies were white and round, with neat edges, smooth and wet surface, and showed β-hemolysis.Gram staining was positive, and the bacteria were arranged in chains of different lengths, single or in pairs.VP test and CAMP were positive, and enzyme contact test was negative, it could ferment trehalose, which accorded with the typical characteristics of S.agalactis.A total of 14 fish-derived S.agalactiae were isolated and identified.The serotypes of the 14 isolated strains were all type Ⅰa.The detection rates of virulence genes cylE, sodA and gapC were all 100%, but none of the virulence gene scpB was detected.The resistance rates of the isolated strains to sulfisoxazole, gentamicin and kanamycin were all 100%, and the resistance rates to streptomycin, neomycin and methotrexate were all more than 70%.The isolates were all multi-drug-resistant strains, and 78.57% of the strains were resistant to more than 6 kinds of drugs.【Conclusion】 This study clarified the serotype, virulence gene and drug resistance characteristics of tilapia S.agalactis in Zhangzhou, so as to provide reference for further study on the pathogenic mechanism and effective clinical prevention and control measures of S.agalactis in tilapia.

Key words: Streptococcus agalactiae; serotype; virulence gene; antibiotic resistance

CLC Number:

S852.61⁺1

YUAN Cheng, CHEN Huaijun, YUAN Sheng, WEN Huamao, CHEN Zhiyi, DONG Hongyan, FENG Qi, GUO Changming. Isolation, Identification and Study on Virulence Gene and Drug Resistance of Streptococcus agalactiae from Tilapia in Zhangzhou City, Fujian Province[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3655-3664.

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Isolation, Identification and Study on Virulence Gene and Drug Resistance of Streptococcus agalactiae from Tilapia in Zhangzhou City, Fujian Province

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