4株Ⅰ亚群禽腺病毒分离株对SPF雏鸡的致病性研究

doi:10.16431/j.cnki.1671-7236.2022.03.025

Abstract

Abstract: 【Object】 The aim of the experiment was to explore the pathogenicity of subgroup Ⅰ Fowl adenovirus (FAdV) isolates GDDL-4(FAdV-4), GDB3-8a(FAdV-8a), GDT08-8b(FAdV-8b) and GDWYT-11(FAdV-11) to SPF chicks, and develop more effective prevention measures.【Method】 The challenge (GDDL-4, GDB3-8a, GDT08-8b and GDWYT-11) and control groups were established, the challenge groups were injected with 0.2 mL 10⁵ TCID₅₀virus into leg muscles, and the control group was injected with equal volume of PBS.The clinical symptoms, autopsy lesions, histopathology of SPF chicks were observed, the detoxification and viral load of SPF chicks were detected.【Result】 The results showed that 4 target strains could all cause depressed, emaciated and clustered.Pathologic autopsy showed that severe hepatitis-hydropericardium syndrome, especially GDDL-4, with mortality rate of 85%, the overall mortality rate was 0 to 85%.The results of detoxification analysis showed that GDDL-4 and GDB3-8a groups had high titer detoxification (≥ 10⁴ copies/μL), while GDT08-8b and GDWYT-11 groups could only detect low titer detoxification (≤ 10³ copies/μL).The organ index showed that there were no significant difference between GDT08-8b and control groups, but the other challenge groups could cause organ swelling or atrophy to varying degrees.The histopathology sections showed that the structures of liver, kidney and bursa of Fabricius were destroyed, it showed that a large number of lymphocytes infiltrated into liver cells, the renal interstitial congestion and hemorrhage, and the lymphocyte necrosis of bursa of Fabricius.The results of viral load analysis showed that GDDL-4 and GDWYT-11 groups had the highest viral titers in liver, while GDB3-8a and GDT08-8b groups had the highest viral titers in duodenum.【Conclusion】 The results indicated that the mortality of subgroup Ⅰ FAdV was high.4 strains of isolates could cause the pathological changes of inclusion body hepatitis in liver of chicks, and could be repeatedly detoxified.Moreover, the isolates had different affinity to different tissues and organs, with the highest viral load in liver and duodenum.

Key words: Fowl adenovirus (FAdV); SPF chicks; autopsy lesions; histopathology; detoxification; viral load

CLC Number:

S852.65⁺9.1

XIE Zimin, ZENG Fancong, FU Yuming, JIANG Hanyu, YANG Huihu, KE Junhong, LUO Rui, LIANG Zhaoping, ZHANG Xuelian, HUANG Shujian. Study on Pathogenicity of 4 Strain of Subgroup Ⅰ Fowl Adenovirus Isolates to SPF Chicks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1032-1040.

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Study on Pathogenicity of 4 Strain of Subgroup Ⅰ Fowl Adenovirus Isolates to SPF Chicks

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