抗鸡球虫药常山口服液对小鼠的急性毒性作用评价

doi:10.16431/j.cnki.1671-7236.2016.10.031

Abstract

Abstract:

The purpose of this study was to evaluate drug safety of Dichroa febrifuga oral liquid (DFOL),and the acute toxicity test of DFOL was established in mice based on modified Karber's method and dose increasing method,and histopathological examination had been applied to explore the main target organs of its toxic injury.The dosage was determined by preliminary experiment,a total of 60 Kunming mice were selected and divided randomly into one control group and five experimental groups,DFOL was administered by intragastric gavage at five different doses (1.00,1.40,1.96,2.74,3.84 g/(kg·BW)),and observed for 7 days continuously,the LD₅₀ value and 95% confidence interval of LD₅₀ were determined by the modified Karbers method.It showed that LD₅₀was 2.0961 g/(kg·BW) and its 95% confidence interval was 1.7414 to 2.5429 g/(kg·BW).The liver and kidney of some mice in 1.96,2.74 and 3.84 g/(kg·BW) drug groups appeared severe edema,congestion and white necrotic foci by necropsy.Acute liver injury was found in mice by histopathological examination and the liver cell showed degenerative change,kidney damage showed renal congestion,interstitial edema,renal tubule epithelial cloudy swelling and degeneration.The liver was damaged seriously in the main organ and the main toxic organ of DFOL was liver.The test showed that the toxicity of DFOL was lower and it had high security in a conventional doses for clinical anti-coccidiosis application,however,high dose,long course of medication had toxicity,and the toxicity of DFOL should be analyzed and evaluated combining with long-term toxicity tests and many other inspection results.

Key words: Dichroa febrifuga oral liquid (DFOL); acute toxicity; tissue pathology; target organ

CLC Number:

S859.8

WANG Ling, GUO Zhi-ting, ZAHNG Xiao-song, LIN Chun-quan, LUO Xiao-qin, YANG Feng, YANG Zhen. Study on Acute Toxicity Test of Anticoccidial Oral Liquid from Dichroa febrifuga in Mice[J]. , 2016, 43(10): 2724-2729.

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Study on Acute Toxicity Test of Anticoccidial Oral Liquid from Dichroa febrifuga in Mice

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