沙冬青种子总黄酮提取纯化与急性毒性及抗氧化活性研究

doi:10.16431/j.cnki.1671-7236.2019.03.036

Abstract

Abstract:

This experiment was conducted to explore the purification and the acute toxicity and the antioxidant activity of total flavonoids from the seeds of Ammopiptanthus.The total flavonoids from the seeds of Ammopiptanthus was extracted using ultrasonic-assisted extraction method.The content of total flavonoids from the seeds of Ammopiptanthus was measured with AlCl₃-CH₄O coloration method.The acute toxicity test of total flavonoids was evaluated in mice.The Fenton system of hydroxyl radicals (·OH) produced in vitro and the superoxide radicals (O₂^-) produced by pyrogallol autoxidation were used to determine the scavenging effect of total flavonoids on radicals.Besides,the effects of total flavonoids on serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in aging model mice were measured.The results showed that the precision (RSD=0.78%),reproducibility (RSD=0.52%),stability (RSD=0.65%) and recovery rate (99.140%) of the method for determination of total flavonoids from the seeds of Ammopiptanthus were in the range of error,and the contain of total flavonoids was 86.780%.The total flavonoids from the seeds of Ammopiptanthus belonged to a non-toxic medicine.The total flavonoids from the seeds of Ammopiptanthus had good scavenging ability to ·OH and O₂^-.In the group of 1 250 μg/mL,there were the best scavenging effects of ·OH and O₂^-,and the clearance rates reached 96.45% and 50.39%,respectively.The total flavonoids could extremely significantly increase the activity of SOD in the serum compared with aging model mice (P<0.01).In the group of 150 mg/(kg·d) total flavonoids,there was the most significant increase of the activity of SOD in the serum,and the activity reached 102.42 U/mL.Furthermore,the total flavonoids could extremely significantly reduce the content of MDA in the serum compared with aging model mice (P<0.01).In the group of 150 mg/(kg·d) total flavonoids,there was the most significant reduction in the content of MDA in the serum.The results suggested that the total flavonoids from the seeds of Ammopiptanthus had good antioxidant activity and scavenging ability on radicals and repair the immune function of aging model mice.

Key words: total flavonoids from the seeds of Ammopiptanthus; purification; acute toxicity; radicals; SOD; MDA

CLC Number:

S853.7

JIN Ye, LIANG Pengfei, FANG Mei, ZHANG Jianan, JIA Ning, DENG Kang, HUANG Weikuan. Extraction,Purification,Acute Toxicity and Antioxidant Activity of Total Flavonoids from Seeds of Ammopiptanthus[J]. , 2019, 46(3): 940-948.

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Extraction,Purification,Acute Toxicity and Antioxidant Activity of Total Flavonoids from Seeds of Ammopiptanthus

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