1株黄曲霉颉颃菌的鉴定及其抑菌活性成分分析

doi:10.16431/j.cnki.1671-7236.2022.05.023

Abstract

Abstract: 【Objective】 This study was aimed to screen the antagonistic bacteria of Aspergillus flavus,and explore its optimal culture conditions and effective antibacterial components,so as to provide a new theoretical basis for the application of biological control methods to inhibit Aspergillus flavus pollution in practical production.【Method】 The strains to be tested were screened and identified by plate confrontation method and molecular biology identification method.Taking the inhibition rate as the measurement standard,the optimal culture temperature,culture time,inoculation amount,rotating speed,pH and liquid loading of Aspergillus flavus antagonistic bacteria were determined.In addition,the protein was extracted from the sterile fermentation supernatant of antagonistic bacteria by ammonium sulfate precipitation method,and the effect of the crude extract on the growth of Aspergillus flavus was observed.Then,the crude protein was further purified by ion exchange chromatography and gel filtration chromatography,and mass spectrometry sequencing and sequence comparative analysis were performed to screen and identify the effective antibacterial components produced by antagonistic bacteria.【Result】 A strain of Bacillus amyloliquefaciens B10 was screened (strain preservation No.CCTCCNO:M2018353).The optimum culture conditions were as follow:Temperature was 40 ℃,culture time was 36 h,inoculation amount was 3%,rotating speed was 120 r/min,pH was 6.0,liquid volume was 30 mL,and the maximum inhibition rate was 46.56%.In addition,the crude protein could effectively inhibit the growth of Aspergillus flavus and destroy the normal morphology of mycelium.Eight possible effective antibacterial components such as chitin binding protein,chitosanase and glucanase had been selected.【Conclusion】 A strain of Bacillus amyloliquefaciens B10,which inhibited the normal growth and development of Aspergillus flavus,was isolated and identified and its various protein components had significant antibacterial activity against Aspergillus flavus.

Key words: Aspergillus flavus; Bacillus amyloliquefaciens; screening and identification; bacteriostatic protein; isolation and purification

CLC Number:

S852.66

LI Tianxi, DANG Meng, LONG Miao. Identification of an Antagonistic Strain of Aspergillus flavus and Preliminary Analysis of Its Antibacterial Components[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1828-1839.

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Identification of an Antagonistic Strain of Aspergillus flavus and Preliminary Analysis of Its Antibacterial Components

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