饲粮中添加地衣芽孢杆菌和解淀粉芽孢杆菌对断奶仔猪生长性能及血液指标的影响

doi:10.16431/j.cnki.1671-7236.2022.07.012

Abstract

Abstract: 【Objective】 This study was conducted to investigate the effects of Bacillus licheniformis and Bacillus amyloliquefaciens supplementation on the growth performance,blood routine,serum biochemical indicators and antioxidant and inflammation indexes of weaned piglets.【Method】 A total of 108 28-day-old weaned piglets (Duroc×Landrace×Yorkshire) were selected and divided into 3 groups according to body weight,with 6 replicates per group and 6 piglets per replicate.The piglets in control group were fed with basal diet,and the other two groups were supplemented with 1 g/kg Bacillus licheniformis or 1 g/kg Bacillus amyloliquefaciens in the basal diet.The experiment lasted for 28 days.At the 14th and 28th days,fasting piglets were weighed and piglets intake were calculated,two piglets were selected from each replication,and the plasma samples were collected from the anterior vena cava,the blood parameters were detected by hematology analyzer and kit.【Result】 There were no significant difference in average daily gain (ADG),average daily feed intake (ADFI) and feed/gain ratio (F/G) among three experiment groups (P>0.05).Compared with control group,the diarrhea rate of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased (P＜0.05).At the 14th day,the percentage of blood lymphocytes percentage of weaned piglets in Bacillus licheniformis and Bacillus amyloliquefaciens groups were significantly increased,while the contents of interleukin-2 (IL-2) and malondialdehyde (MDA),and the activities of lactate dehydrogenase and aspartate aminotransferase were significantly decreased (P＜0.05).At the 14th day,the serum alkaline phosphatase activity of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased,while the hemoglobin was significantly increased (P＜0.05).At the 28th day,the content of IL-2 of weaned piglets in Bacillus amyloliquefaciens group was significantly decreased,while the contents of total protein and globulin were significantly increased (P＜0.05).【Conclusion】 The supplement of Bacillus licheniformis or Bacillus amyloliquefaciens had no outstanding effects on the growth performance,but it could improve the blood biochemical indicators and protect the liver of weaned piglets,and the supplement of Bacillus amyloliquefaciens could also improve the metabolism of piglets and decrease the diarrhea rate of piglets.

Key words: Bacillus licheniformis; Bacillus amyloliquefaciens; weaned piglets; growth performance; blood indicators

CLC Number:

S816.7

CHEN Xueying, WANG Huisheng, WU Jie, DENG Bo, DIAO Xinping. Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2547-2556.

References

[1] GASKINS H R,COLLIER C T,ANDERSON D B.Antibiotics as growth promotants:Mode of action[J].Animal Biotechnology,2002,13(1):29-42.
[2] SHEN D S,TAO X Q,SHENTU J L,et al.Residues of veterinary antibiotics in pig feeds and manures in Zhejiang province[J].Advanced Materials Research,2014,1010-1012:301-304.
[3] 肖怡,陶大勇,赵明明,等.地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响[J].动物营养学报,2016,28(2):515-523. XIAO Y,TAO D Y,ZHAO M M,et al.Effects of Baclicus lincheniformis on methane emission,digestion and metabolism of mutton sheep[J].Chinese Journal of Animal Nutrition,2016,28(2):515-523.(in Chinese)
[4] 栾素军,邢焕,孙永波,等.解淀粉芽孢杆菌在畜禽养殖中的应用研究进展[J].中国畜牧兽医,2016,43(10):2615-2620. LUAN S J,XING H,SUN Y B,et al.Research progress on Bacillus amyloliquefaciens in animal breeding industry[J].China Animal Husbandry & Veterinary Medicine,2016,43(10):2615-2620.(in Chinese)
[5] WANG Y,DU W,LEI K,et al.Effects of dietary Bacillus licheniformis on gut physical barrier,immunity,and reproductive hormones of laying hens[J].Probiotics and Antimicrobial Proteins,2017,9(3):292-299.
[6] LIU X L,YAN H,LV L,et al.Growth performance and meat quality of broiler chickens supplemented with Bacillus licheniformis in drinking water[J].Asian-Australasian Journal of Animal Sciences,2012,25(5):682-689.
[7] 戴利铭,李岚岚,刘一贤,等.解淀粉芽孢杆菌生防菌BS-3全基因组测序及生物信息分析[J].微生物学通报,2021,48(6):2073-2088. DAI L M,LI L L,LIU Y X,et al.Whole genome sequencing and genomics analysis of Bacillus amyloliquefaciens BS-3 with biocontrol activity[J].Microbiology China,2021,48(6):2073-2088.(in Chinese)
[8] ALFONZO A,PICCOLO S L,CONIGLIARO G,et al.Antifungal peptides produced by Bacillus amyloliquefaciens AG1 active against grapevine fungal pathogens[J].Annals of Microbiology,2012,62(4):1593-1599.
[9] 曹广添.解淀粉芽孢杆菌的抑菌机理及对肉鸡生长代谢和盲肠微生物菌群的影响[D].杭州:浙江大学,2015. CAO G T.Effects of Bacillus amyloliquefaciens on the growth,metabolites and diversity of cecalmicroflora in broilers and its antibacterial mechanism[D].Hangzhou:Zhejiang University,2015.(in Chinese)
[10] JI J,HU S L,ZHENG M Z,et al.Bacillus amyloliquefaciens SC06 inhibits ETEC-induced pro-inflammatory responses by suppression of MAPK signaling pathways in IPEC-1 cells and diarrhea in weaned piglets[J].Livestock Science,2013,158(1-3):206-214.
[11] ZONG X,WANG T H,LU Z Q,et al.Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance,blood indexes,and intestinal barrier function of weanling piglets[J].Livestock Science,2019,220:137-142.
[12] 邓文,徐彬,孙全友,等.地衣芽孢杆菌和低聚木糖对断奶仔猪生长性能、肠道形态和通透性及粪便微生物的影响[J].中国饲料,2019,15:40-44. DENG W,XU B,SUN Q Y,et al.Effects of Bacillus licheniformis and xylooligosaccharides on the performance,intestinal morphology and permeability,and fecal microbes of weaned piglets[J].China Feed,2019,15:40-44.(in Chinese)
[13] WIZNA H,ABBAS Y,RIZAL A,et al.The effect of supplementation of micro nutrient on nutrient rice bran which fermented by Bacillus amyloliquefaciens[J].Pakistan Journal of Nutrition,2012,11(5):439-443.
[14] WANG H,YANG L,PING Y G,et al.Engineering of a Bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions[J].PLoS One,2016,11(1):e0146373.
[15] LEI X J,RU Y J,ZHANG H F.Effect of Bacillus amyloliquefaciens-based direct-fed microbials and antibiotic on performance,nutrient digestibility,cecalmicroflora,and intestinal morphology in broiler chickens[J].Journal of Applied Poultry Research,2014,23(3):486-493.
[16] 尹会方,姚志翔,杨守深,等.丁酸梭菌和地衣芽孢杆菌对断奶仔猪生长性能及血液指标的影响[J].中国饲料,2019,20:33-36. YIN H F,YAO Z X,YANG S S,et al.Effects of Clostridium butyricum,Bacillus licheniformis on the growth performance and blood indexes of piglets[J].China Feed,2019,20:33-36.(in Chinese)
[17] 徐小明,白建勇,宦海琳,等.地衣芽孢杆菌对发酵床饲养仔猪生长性能、消化酶活性及肠道主要菌群数量的影响[J].中国畜牧兽医,2015,42(4):923-928. XU X M,BAI J Y,HUAN H L,et al.Effect of Bacillus licheniformis on growth performance,digestive enzymes and the number of intestinal main microbial flora of piglets raised in fermentation bed[J].China Animal Husbandry & Veterinary Medicine,2015,42(4):923-928.(in Chinese)
[18] 廖柱,蔡旭旺,谢红涛,等.添加植物乳杆菌与枯草芽孢杆菌对仔猪生长性能和血清生化指标的影响[J].饲料研究,2021,44(21):32-35. LIAO Z,CAI X W,XIE H T,et al.Effect of Lactobacillus plantarum and Bacillus subtilis supplementation on growth performance and serum biochemical indices of piglets[J].Feed Research,2021,44(21):32-35.(in Chinese)
[19] PEET-SCHWERING C,VERHEIJEN R,JRGENSEN L,et al.Effects of a mixture of Bacillus amyloliquefaciens and Bacillus subtilis on the performance of growing-finishing pigs[J].Animal Feed Science and Technology,2020,261:114409.
[20] STROMPFOVA V,MARCINAKOVA M,SIMONOVA M,et al.Enterococcus faecium EK13--An enterocin A-Producing strain with probiotic character and its effect in piglets[J].Anaerobe,2006,12(5/6):242-248.
[21] 张闻桐,郭亮,李杰,等.益生菌与宿主疾病关系的研究进展[J].微生物学杂志,2020,40(4):90-97. ZHAGN W T,GUO L,LI J,et al.Advances in relationship between probiotics and host disease[J].Journal of Microbiology,2020,40(4):90-97.(in Chinese)
[22] 王淑梅,邸维,妥彦峰,等.益生菌的免疫调控作用研究进展[J].粮食与油脂,2021,34(5):23-26. WANG S M,DI W,TUO Y F,et al.Research progress on immune regulation of probiotics[J].Cereals & Oils,2021,34(5):23-26.(in Chinese)
[23] 冯宝宝,方伟,封飞飞,等.屎肠球菌SF68对断奶仔猪生长性能、血常规和血清生化指标及抗氧化性能的影响[J].动物营养学报,2018,30(3):1125-1135. FENG B B,FANG W,FENG F F,et al.Effects of Enterococcus faecalis SF68 on growth performance,blood routine and serum biochemical indexes and antioxidant ability of weaned piglets[J].Chinese Journal of Animal Nutrition,2018,30(3):1125-1135.(in Chinese)
[24] 郭军蕊,董晓芳,佟建明.枯草芽孢杆菌CGMCC 1.921对蛋鸡生产性能、血常规指标、血清生化指标及免疫球蛋白含量的影响[J].动物营养学报,2017,29(2):465-478. GUO J R,DONG X F,TONG J M.Effects of Bacillus subtilis CGMCC 1.921 on performance,routine blood parameters,serum biochemical parameters and immunoglobulin contents of laying hens[J].Chinese Journal of Animal Nutrition,2017,29(2):465-478.(in Chinese)
[25] 张安荣,刘娇,陈志敏,等.饲粮添加枯草芽孢杆菌对肉仔鸡生长性能、血清生化指标和胫骨指标的影响[J].动物营养学报,2020,32(9):4123-4131. ZHANG A R,LIU J,CHEN Z M,et al.Effect of dietary Bacillus subtilis on growth performance,serum biochemical indices and tibia index of broilers[J].Chinese Journal of Animal Nutrition,2020,32(9):4123-4131.(in Chinese)
[26] 卢佳伟,王金刚,高琴,等.不同益生菌对断奶山羊生长性能、血清生化指标以及粪便菌群的影响[J].动物营养学报,2021,33(5):2752-2764. LU J W,WANG J G,GAO Q,et al.Effects of different probiotics on growth performance,serum biochemical indexes and fecal microflora of weaned goats[J].Chinese Journal of Animal Nutrition,2021,33(5):2752-2764.(in Chinese)
[27] 袁丰华,林黑着,李卓佳,等.地衣芽孢杆菌对尖吻鲈血液生理生化指标的影响[J].南方水产,2009,5(2):45-50. YUAN F H,LIN H Z,LI Z J,et al.Effects of dietary Bacillus licheniformis on blood physiological-biochemical indices in cultured Latescalarifer[J].South China Fisheries Science,2009,5(2):45-50.(in Chinese)
[28] JONES J G.Hepatic glucose and lipid metabolism[J].Diabetologia,2016,59(6):1098-1103.
[29] 闫蕾,赵彩彦.肝脏--一个新的免疫器官[J].国际内科学杂志,2009,36(4):206-210. YAN L,ZHAO C Y.Liver--A new immune organ[J].International Journal of Internal Medicine,2009,36(4):206-210.(in Chinese)
[30] 唐武,蔡巧利,蒋慧姣,等.饲粮添加益生菌和合生元对环江香猪生长性能及血浆生化参数的影响[J].动物营养学报,2021,33(1):546-552. TANG W,CAI Q L,JIANG H J,et al.Effects of detary supplementation with probiotics and synbiotics on growth performance and plasma biochemical parameters of Huanjiang Mini-piglets[J].Chinese Journal of Animal Nutrition,2021,33(1):546-552.(in Chinese)
[31] LI X T,LV Z M,CHEN J,et al.Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B₁[J].Food and Chemical Toxicology,2021,151:112-124.
[32] 张立永,任海洋.地衣芽孢杆菌对雏鸡生长性能、肠道结构及血清生化指标的影响[J].黑龙江畜牧兽医,2019,12:113-116. ZHANG L Y,REN H Y.Effects of Bacillus licheniformis on growth performance,intestinal structure and serum biochemical indexes of chicks[J].Heilongjiang Animal Science and Veterinary Medicine,2019,12:113-116.(in Chinese)
[33] 尹清强,李小飞,常娟,等.微生态制剂对哺乳和断奶仔猪生产性能的影响及作用机理研究[J].动物营养学报,2011,23(4):622-630. YIN Q Q,LI X F,CHANG J,et al.Probiotics:Effects on performance and mechanisms of action in suckling and weaner piglets[J].Chinese Journal of Animal Nutrition,2011,23(4):622-630.(in Chinese)
[34] WANG Y,WU Y P,WANG B K,et al.Effects of probiotic Bacillus as a substitute for antibiotics on antioxidant capacity and intestinal autophagy of piglets[J].AMB Express,2017,7(1):52.
[35] 应志雄,李悦,张昊,等.解淀粉芽孢杆菌对免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响[J].中国兽医学报,2018,38(8):1609-1615. YING Z X,LI Y,ZHANG H,et al.Effects of Bacillus amyloliquefaciens on growth performance and liver antioxidant function of immunosuppressed broilers[J].Chinese Journal of Veterinary Medicine,2018,38(8):1609-1615.(in Chinese)
[36] 杨家军,钱坤,章薇,等.地衣芽孢杆菌对肉鸡生产性能、抗氧化及营养物质代谢的影响研究[J].中国兽医学报,2014,34(5):736-739. YANG J J,QIAN K,ZHANG W,et al.Effect of supplementary Bacillus licheniformis on growth performance,antioxidation and nutrient metabolism in Partridge shank chicken[J].Chinese Journal of Veterinary Medicine,2014,34(5):736-739.(in Chinese)
[37] 贺驭,孔祥峰,王振勇,等.益生菌抗氧化机制的研究进展[J].中国家禽,2017,39(24):41-44. HE Y,KONG X F,WANG Z Y,et al.Advances in antioxidant mechanisms of probiotics[J].China Poultry,2017,39(24):41-44.(in Chinese)
[38] 刘煜珺,张雨晴,高原,等.乳杆菌胞外多糖抗氧化活性研究[J].中国食品学报,2019,19(6):21-35. LIU Y J,ZHAGN Y Q,GAO Y,et al.Studies on the antioxidative activity of exopolysaccharide from Lactobacillus strains[J].Journal of Chinese Institute of Food Science and Technology,2019,19(6):21-35.(in Chinese)
[39] 熊传帅,黎力之,周建旭,等.精氨酸对动物肠道炎症的影响及其在畜牧生产上的应用研究进展[J].中国畜牧杂志,2021,57(12):20-25. XIONG C S,LI L Z,ZHOU J X,et al.Advances in effects of arginine on intestinal inflammation in animals and its application in animal husbandry[J].Chinese Journal of Animal Science,2021,57(12):20-25.(in Chinese)

Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Yanghui, XU Tao, ZHANG Shuxian, LIU Ke, JIA Tianqing, CHEN Ning, YIN Junliang, XI Linqiao, WANG Dong, ZHOU Xiaoling. Effect of Replacing Wheat Straw with Cumin Straw on Growth Performance, Slaughter Performance and Plasma Biochemical Parameters of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2296-2304.
[2]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[3]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[4]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[5]	CHEN Xi, LI Xinyi, CHEN Fashun, DUAN Yong, DONG Ling, WU Shujie, FAN Jiancheng, XIANG Bin, YANG Liangyu, ZHANG Limei. Study on the Probiotic Properties and Antimicrobial Activity of 5 Strains of Bacillus amyloliquefaciens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2082-2091.
[6]	REN Jiangang, GUAN Peng, HUANG Jianzhi, SHENG Yueyun, BAO Tiezhu, MA Zhiping, QIAN Wenxi. Effects of Dietary Energy Level on Growth-fattening Performance and Serum Biochemical Indices of Angus Bulls [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 966-976.
[7]	XIONG Taidi, JIANG Shouqun, ZHANG Huihua, ZHU Cui. Research Progress on Transportation Stress and Its Mitigation Measures in Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 977-988.
[8]	LI Bin, SHI Zhengling, ZENG Ziyou, HUANG Yingyan, LI Lin, DU Zilai, JI Yifan, WANG Di, YU Jie, MEI Shaofeng. Effects of Chicory on Growth Performance,Slaughter Traits and Meat Quality of Mahuang Broilers with Different Gender [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 500-511.
[9]	WANG Yuan, WANG Wenwen, AN Xiaoping, QI Jingwei. Effects of Dietary Fermented Soybean Meal Replacement on Growth Performance, Digestive Enzyme,and Microbita Composition in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 115-124.
[10]	LIN Xiajing, ZHENG Chuntian, XI Pengbin, CHENG Zhonggang, DING Fayuan, RUAN Dong, WANG Yibing, JIANG Shouqun. Dietary Threonine Requirement of Yellow-feathered Broilers Aged From 22 to 42 Days [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 134-142.
[11]	WO Yeqianli, GAO Duo, MA Fengtao, JIN Yuhang, LIU Zhuo, SUN Peng. Effects of Two Different Organic Zinc Sources Supplementation on Growth Performance and Zinc Metabolism of Newborn Heifers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3382-3390.
[12]	WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.
[13]	CHEN Lihong, ZHAO Mengdi, LIU Keyuan, NING Haoran, CUI Kai, LI Guangyu, SUN Weili. Study on Optimum Dietary Metabolizable Energy Level of Male Pheasants from 13 to 17 Weeks of Age [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2943-2952.
[14]	PENG Kai, CHEN Bing, ZHAO Hongxia, WANG Lijuan, ZHU Xifeng, HUANG Wen. Effects of Condensed Tannins on Growth Performance,Body Composition,Apparent Digestibility of Nutrients,and Intestinal Morphology of Lateolabtax maculatus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2953-2960.
[15]	REN Jianming, JI Peng, ZHANG Xiaosong, WEI Yanming, ZHU Xiaopeng, ZHU Yueming. Effects of Jianpi Xiaodao Chinese Herbal Compound on Growth Performance, Rumen Fermentation Parameters and Serum Indexes of 6-month-old Simmental Crossbred Cattles [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2593-2600.