5株解淀粉芽孢杆菌的益生特性及抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2023.05.035

Abstract

Abstract: 【Objective】 This study was aimed to explore the growth curve,pH,bile salt tolerance,antimicrobial activity,antibiotic sensitivity and other characteristics of 5 strains of self screening Bacillus amyloliquefaciens(YN-BA1,YN-BA2,YN-BA3,YN-BA4 and YN-BA5),and provide theoretical basis for the development of microecological agents in aquaculture.【Method】 In this test,the growth curve of Bacillus amyloliquefaciens was determined by turbidimetry.The 5 strains of Bacillus amyloliquefaciens were inoculated into LB broth prepared with hydrochloric acid,pH 2.0,3.0,4.0 and 7.0 for 2 h,and cultured 5 h in an environment with 0.3% bile salt concentration to evaluate their tolerance in artificial gastric juice intestinal juice.The antibacterial activity in vitro was determined by agar drilling method,and the antibiotic sensitivity was determined by KB paper method.【Result】 The growth rates of 5 strains of Bacillus amyloliquefaciens increased rapidly and entered the logarithmic growth phase after the slow growth 0-2 and 6-8 h.After 36 h,the growth decreased and entered the recession period.The 5 strains had different abilities of acid resistance and bile salt tolerance.Among them,the survival rates of YN-BA2 strain at pH 2.0,3.0 and 4.0 were 42.10%,51.50% and 74.67%,respectively,which were higher than the other 4 strains.After being treated for 5 h at 0.3% bile salt concentration,the survival rate of YN-BA2 was 51.00%,which was higher than the other 4 strains,indicating that YN-BA2 strain had the highest tolerance.The 5 strains of Bacillus amyloliquefaciens had antibacterial effect on Salmonella Enteritidis YN-S1 and YN-S2,Salmonella Pullorum YN-SP9 and YN-SP10,and Escherichia coli DO157.YN-BA2 had high sensitivity to Salmonella Pullorum YN-SP10.The other 4 strains of Bacillus amyloliquefaciens were moderately sensitive to intestinal pathogens preserved in the laboratory,indicating that YN-BA2 had the best antibacterial effect.The 5 strains of Bacillus amyloliquefaciens were resistant to tetracycline (except for YN-BA5) and norfloxacin,ofloxacin,peillin G and cephalexin to varying degrees,and were sensitive to erythromycin,kanamycin (except for YN-BA5),levofloxacin,ciprofloxacin,gentamicin,azithromycin (except for YN-BA1),ampicillin and lincomycin.【Conclusion】 Bacillus amyloliquefaciens could be activated rapidly,had good tolerance to bile salt and pH,had certain inhibitory effects on Escherichia coli,Salmonella Pullorum and Salmonella Enteritidis,and was resistant to tetracycline and norfloxacin.Therefore,Bacillus amyloliquefaciens had the potential to be used in the development of microecological agents for livestock and poultry production.

Key words: Bacillus amyloliquefaciens; probiotic properties; antimicrobial activity

CLC Number:

S816.7

CHEN Xi, LI Xinyi, CHEN Fashun, DUAN Yong, DONG Ling, WU Shujie, FAN Jiancheng, XIANG Bin, YANG Liangyu, ZHANG Limei. Study on the Probiotic Properties and Antimicrobial Activity of 5 Strains of Bacillus amyloliquefaciens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2082-2091.

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Study on the Probiotic Properties and Antimicrobial Activity of 5 Strains of Bacillus amyloliquefaciens

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