一株解淀粉芽孢杆菌的特性及抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2018.05.016

Abstract

Abstract:

The aim of the experiment was to analyze the growth curve,acid resistance and bile acid salt tolerance of a strain of Bacillus amyloliquefaciens from rumen,and to determine the bacteriostasis activity and antibacterial activity stability of the fermented product of the strain.The growth curve of the strain was determined by growth rate method,the viable count method was used to determine the acid resistance and bile acid salt tolerance,and the antibacterial activity and antibacterial activity stability were determined by the Oxford cup method.The results showed that the bacteria entered the logarithmic growth phase after 4 h of growth and tended to be stable after 20 h of growth and entered the stable growth phase.At the same time,the bacteria showed good acid resistance,and the survival rates were 23.8% to 90.6% at pH 3.0 to 7.0;And the bile acid salt tolerance was better,and the survival rates were 64.2% to 83.6% in bile acid concentration of 0.1% to 0.4%.Bacteria such as Escherichia coli,Vibrio parahemolyticus,Sarcina lutea and Aerogenes,and fungi such as Aspergillus niger and Rhizopus nigricans, as bacteriostatic indicator bacteria for antibacterial test,and the results showed that the fermentation products of the Bacillus amyloliquefaciens showed antibacterial activity,and its antibacterial active substance had good heat resistance and acid resistance.In this experiment,the properties of Bacillus amyloliquefaciens were studied and it had the properties of acid resistance and ile acid salt tolerance;The bacteriostatic activity and bacteriostasis stability were good,and the bacterium had acid and alkali resistance and high temperature resistance.

Key words: Bacillus amyloliquefaciens; biological characteristics; antimicrobial activity; acid-base resistance

CLC Number:

S816.7

ZHANG Xue, TAN Shiyao, QIAN Siriguleng, WANG Hongying. Study on the Characteristics and Antibacterial Activity of a Bacillus amyloliquefaciens[J]. , 2018, 45(5): 1251-1257.

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Study on the Characteristics and Antibacterial Activity of a Bacillus amyloliquefaciens

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