几种商用益生芽孢杆菌特性的比较研究

doi:10.16431/j.cnki.1671-7236.2018.05.015

Abstract

Abstract:

The probiotic characteristics of commercial Bacillus products were compared using screening techniques in this study.Fifteen samples of medicinal (Y1-Y6) and feed sources (S7-S15) commercial Bacillus probiotics were collected to isolate and identify the Bacillus spp.,and their probiotic characteristics were compared including enzymic properties of amylase,cellulase and protease,antimicrobial activities,tolerance tests to acid and 0.3% bile salts, antibiotics sensitivity,oxygen-consumptions rate and growth rate.The results showed that all the 15 strains could produce amylase,cellulase and protease with different enzyme capacity,and that produced by S9 strain was the highest.All strains could inhibit the strains of Staphylococcus aureus and Micrococcus luteus.Only 7 strains (Y2,Y6,S7,S9,S10,S11 and S12) showed antimicrobial activity against E.coli.And the S9 strain showed the highest antimicrobial activity against all the three indicator bacteria.The vegetative cells of S9,Y2 and S8 had a strong tolerance to low acid environment and the survival rates of S9 and Y2 strains in 0.3% bile salt environment were more than 90%.Three strains (S10,S12 and S13) were resistant to erythrocin and S10 strain was resistant to tetracycline.The S9 strain grew faster with higher rate of oxygen consumption.In conclusion,S9 strain identified as Bacillus amyloliquefaciens showed better beneficial characteristics in all the 15 commercial probiotic Bacillus strains,and the study results could provide references for the selection and evaluation of probiotics of Bacillus.

Key words: Bacillus; probiotics; characterization comparison

CLC Number:

S816.7

YANG Kunming, REN Hang, SU Yating, GUO Xiaohua. Comparative Evaluation of Probiotic Characterization of Commercial Bacillus Products[J]. , 2018, 45(5): 1241-1250.

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Comparative Evaluation of Probiotic Characterization of Commercial Bacillus Products

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