1株解淀粉芽孢杆菌B7快速液态发酵优化工艺研究

doi:10.16431/j.cnki.1671-7236.2021.03.002

Abstract

Abstract: In this study,a strain of probiotic Bacillus amyloliquefaciens B7 (B.amyloliquefaciens B7) was used as the research object,in order to improve its level of fermentation under liquid conditions,and to improve the optimum efficiency of spore fermentation.The method of determination of dipicolonic acid (DPA) concentration was proposed to replace the traditional plate spores counting method to explore the feasibility of this method in the optimum process of spore fermentation.The significant factors affecting spore production were first screening through the single-factor experiment.Firstly,the factors that had significant promoting effect on spore production of strain were screened out by single factor test.The optimum combination of peptone,corn powder,rice protein powder and Mn²⁺ was determined by orthogonal experiment.The result showed that the spore concentration was linearly dependent on the fluorescence intensity of DPA in the method,and the optimal medium for higher spore concentration was peptone 20 g/L,corn flour 10 g/L,rice protein powder 20 g/L,Mn²⁺ 1.0 mmol/L.After 48 h of fermentation,spore concentration reached 7.0×10⁹ CFU/mL.Compared with the LB medium and the industrial production medium used in practice,the spore concentration was increased by 3.3 and 2.2 times,respectively.This study provided a rapid method for the determination of spore concentration during fermentation of Bacillus,and a simpler medium with high spore yields was presented for industrial spore fermentation of B.amyloliquefaciens B7 based on the rapid quantification of spore concentration.

Key words: Bacillus amyloliquefaciens; dipicolonic acid; fluorescence intensity; submerged fermentation; optimization

CLC Number:

S816.7

LI Gangping, LONG Zhu, LI Xiaowen, KUANG Shichang, GUO Xiaohua. Study on the Optimization Technology of Rapid Liquid Fermentation of a Strain of Bacillus amyloliquefaciens B7[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 792-800.

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Study on the Optimization Technology of Rapid Liquid Fermentation of a Strain of Bacillus amyloliquefaciens B7

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