中药联合复合益生菌对鸡柔嫩艾美耳球虫的预防作用

doi:10.16431/j.cnki.1671-7236.2023.05.036

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the effect of the combination of traditional Chinese medicine with probiotics on the prevention of Eimeria tenella.【Method】 A total of 180 12-day-old Guinong golden chickens were divided into blank control group,infection group,traditional Chinese medicine group,probiotic group,traditional Chinese medicine + probiotic group and monensin group,with 3 replicates in each group and 10 chickens in each replicate.Blank control group and infection group were treated with fasting normal saline (200 μL/chicken),traditional Chinese medicine group was feed the basal diet with 2% Chinese medicine prescription,probiotics group was treated with fasting compound probiotics (200 μL/chicken),traditional Chinese medicine + probiotics group was treated with fasting compound probiotics (200 μL/chicken) and feed the basal diet with 2% Chinese medicine prescription.Monensin group was feed basal diet with 100 mg/kg monensin.At 14 days of age,except blank control group,all groups were orally administrated with 1×10⁵ oocysts/chicken sporoidized oocysts suspension of Eimeria tenella. The relative weight gain rate and survival rate were measured before inoculation and culturing.Blood samples were collected on days 0,4 and 8 after infection,and serum biochemical,immune and antioxidant indexes were determined by ELASA method.Cecal tissues were collected and histopathological changes were observed.【Result】 The results showed that the relative weight gain rate of each prophylactic administration group was higher than that of infection group,and the number of oocysts per gram of cecum was significantly lower than that of infection group (P<0.05). The relative weight gain rate of traditional Chinese medicine + probiotics group was the highest,the number of oocysts per gram of cecum was the lowest,and the anticoccidial index (ACI) was higher than that of monensin group.Compared with infection group,on days 4 of infection,the contents of serum albumin (ALB) and triglyceride (TG) in traditional Chinese medicine group,probiotic group and traditional Chinese medicine + probiotic group were significantly increased (P ＜0.05),and the content of creatinine (CREA) was significantly decreased (P＜0.05).The contents of total protein (TP),interleukin-2 (IL-2),IL-6 and interferon-γ (IFN-γ) of serum in traditional Chinese medicine group and traditional Chinese medicine + probiotic group were significantly increased (P ＜0.05),and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly decreased (P＜0.05).On days 8 of infection,the contents of TP and ALB of serum in traditional Chinese medicine group,probiotic group and traditional Chinese medicine + probiotic group were significantly increased (P＜0.05),while the content of urea nitrogen (BUN) and AST activity were significantly decreased (P＜0.05).The contents of TG,IL-2,IL-6 and IFN-γ in traditional Chinese medicine group and traditional Chinese medicine + probiotics group were significantly increased (P＜0.05),the contents of CREA and the activity of ALT were significantly decreased (P＜0.05).The results of cecal histopathology showed that the infection group and the probiotics group had obvious pathological damage,while the traditional Chinese medicine group and the traditional Chinese medicine + probiotics group had slight tissue damage.【Conclusion】 This study showed that the combined use of traditional Chinese medicine and probiotics could improve the growth inhibition caused by Eimeria tenella infection,reduce the output of oocysts,improve the immunity and antioxidant capacity of the body,and effectively protect the cecum tissue. This study provided providing reference for the preventio and treatment of coccidiosis.

Key words: chickens; Eimeria tenella; traditional Chinese medicine; probiotics; prevention

CLC Number:

S855.9

JIANG Shanyi, ZHOU Bijun, WEN Ming, WANG Kaigong. Prevention of Traditional Chinese Medicine with Probiotics on Eimeria tenella[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2092-2102.

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Prevention of Traditional Chinese Medicine with Probiotics on Eimeria tenella

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