4种中药单体与丁酸钠诱导猪宿主防御肽表达的协同效果研究

doi:10.16431/j.cnki.1671-7236.2022.10.039

Abstract

Abstract: 【Objective】 This study was conducted to investigate the synergistic effects of Chinese herbal monomers xanthohumol,isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate on the expression of porcine host defense peptide (HDPs).【Method】 Porcine intestinal epithelial cells (IPEC-J2) and porcine alveolar macrophages (3D4/31) were treated with Chinese herbal monomers xanthohumol,isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate alone or together.HDPs gene expression,cytokine gene expression and antibacterial effect of cells on enterotoxigenic Escherichia coli (ETEC) were measured to evaluate the synergistic effects of Chinese herbal monomers and sodium butyrate.【Result】 Xanthohumol and sodium butyrate had no synergistic effect on the expression of HDPs.The expression of pEP2C and pBD3 genes in IPEC-J2 cells was synergistically induced by isorhapontigenin and sodium butyrate (P＜0.01).Deoxyshikonin combined with sodium butyrate could synergically increase the expression of pBD3 and PG1-5 genes in IPEC-J2 cells (P＜0.05;P＜0.01).Treatment with calycosin and sodium butyrate could synergically promote PG1-5,pEP2C,pBD2 and pBD3 genes expression in IPEC-J2 cells (P＜0.05;P＜0.01) and pBD3 gene expression in 3D4/31 cells (P＜0.05).The co-treatment of Chinese herbal monomers and sodium butyrate had no significant cooperative effect on cytokine expression (P＞0.05).Except xanthohumol,other Chinese herbal monomers and sodium butyrate treatment alone or together could improve the antibacterial activity of cells to ETEC.Calycosin and sodium butyrate treatment showed the best synergistic effect on the inhibition of ETEC.【Conclusion】 Chinese herbal monomers isorhapontigenin,deoxyshikonin and calycosin and sodium butyrate had a certain synergistic effect on inducing HDPs expression,improving immune and antibacterial activity of the host.

Key words: Chinese herbal monomers; sodium butyrate; synergy; host defense peptide

CLC Number:

S859.3

CHU Xu, ZHANG Junxia, WANG Jing. Synergistic Effect of Four Chinese Herbal Monomers and Sodium Butyrate on the Expression of Porcine Host Defense Peptide[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4063-4074.

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Synergistic Effect of Four Chinese Herbal Monomers and Sodium Butyrate on the Expression of Porcine Host Defense Peptide

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