Abstract: This experiment was conducted to explore the acute toxicity and hypoglycemic activity in diabetic mice of total flavonoids from Melastoma dodecandrum Lour. (MDF).The mice were intragastrically administered with MDF solution (5.6 mg/(g·BW)) with the maximum gastric volume (40 μL/(g·BW)),solution was administered twice for 24 h.The state of mice in 14 d was recorded.At the end of the test,the levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT),creatinine (Cre) and urea nitrogen (BUN) were measured.The organ index was calculated by weighting the heart,liver,spleen,lung and kidney.The diabetic mouse model was established by intraperitoneal injection of 0.160 mg/(g·BW) streptozotocin (STZ).The mouse were divided into blank group,model group,metformin group (0.6 mg/(g·BW)),MDF high dose,medium dose and low dose groups (1.2,0.8,0.53 mg/(g·BW)).After 21 days of continuous administration,fasting blood glucose (FBG),body mass,feed intake,water intake and 24 h urine protein content of each group of mice were measured.The results showed that the maximum daily dose of MDF was 11.2 mg/(g·BW)).Compared with control group,there was no significant difference in body mass,organ index,and serum AST,ALT,Cre and BUN of the mouse after administration (P>0.05).In the hypoglycemic activity test,compared with the model group,the FBG,water and feed intake of MDF-treated group were significantly or extremely significantly reduced (P<0.05;P<0.01).The above results confirmed that the MDF was safe and non-toxic,it could effectively alleviate the symptoms and decrease blood glucose in diabetic mice.

Key words: Melastoma dodecandrum Lour.; total flavonoids; acute toxicity; diabetes