China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1602-1610.doi: 10.16431/j.cnki.1671-7236.2020.05.035

• Basic Veterinary Medicine • Previous Articles     Next Articles

Acute Toxicity and Subchronic Toxicity Test of Tribonema Powder

WEI Qianhan1, LEI Weiwei1, CHEN Xin1, ZHAO Weiya1, ZHANG Meng1, XIAO Jinzha1, ZHANG Yumei1,2   

  1. 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;
    2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
  • Online:2020-05-20 Published:2020-05-18

Abstract: The purpose of the experiment was to evaluate the feeding safety of Tribonema by the acute toxicity and subchronic toxicity test on rats.5 000 mg/(kg·BW) of Tribonema powder was given to Wistar rats by gavage for acute toxicity test.The rats were fed with 0,1 250,2 500 and 5 000 mg/kg of Tribonema powder for 90 days.The weight and feed consumption of rats were measured every week.The rats were dissected at 45 and 90 days after feeding.The blood routine and biochemical indexes were detected.The organ coefficient was calculated and the main organs were examined by histopathology.The results showed that there was no death in the rats treated with 5 000 mg/(kg·BW) of Tribonema powder.LD50 was more than 5 000 mg/(kg·BW).According to WHO’s toxicity rating of chemicals,Tribonema powder belonged to the actual non-toxic substances.In the subchronic toxicity test,there was no significant difference in the average feed consumption,body weight growth and organ coefficient of rats fed with high,medium and low doses of Tribonema powder after 90 days compared with the control group (P>0.05).There was no significant difference in blood routine indexes and blood biochemical indexes between low dose group and control group (P>0.05).Besides the blood total cholesterol (CHO) and triglyceride (TG) in the high and middle dose groups were significantly lower than those in control group (P<0.05),there was no significant difference in other blood biochemical indexes (P>0.05),but the CHO and TG in the high and middle dose groups were all in the normal biochemical indexes of rats within the scope.No significant changes were found in gross anatomy and HE staining of main organs.The results of this experiment showed that the Tribonema powder belonged to the actual non-toxic substance,the rats were fed with 5 000 mg/kg for 90 days,and no obvious harmful effect was observed,which was expected to be applied in animal feed.

Key words: Tribonema powder; acute toxicity; subchronic toxicity; rat

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