China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (5): 1754-1763.doi: 10.16431/j.cnki.1671-7236.2023.05.003

• Biotechnology • Previous Articles     Next Articles

Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown

PIAN Huifang1, DU Xubin2, LI Yan1, ZHANG Yuchen1, LIU Fei2, YU Debing1   

  1. 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. College of Animal Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2022-10-05 Online:2023-05-05 Published:2023-04-28

Abstract: 【Objective】 This study was amied to obtain the full-length transcriptome database of granulosa cells of small yellow follicle (SYFG),and provide references for further exploring the internal molecular regulation of chicken follicular development.【Method】 The SYFG of Hy-Line variety Brown was sequenced using PacBio high-throughput sequencing platform,and the full-length transcriptome sequence was finally obtained by filtering, clustering and correction.Bioinformatics analysis software was used to compare the full-length transcriptome data for function annotation,transcription factor annotation,coding sequence (CDS) prediction,long non-coding RNA (lncRNA) prediction and screening,simple sequence repeats (SSR) detecting and alternative splicing (AS),and alternative polyadenylation (APA) analysis.【Result】 A total of 52 311 full-length transcripts of SYFG of Hy-Line variety Brown were obtained,and the length was mainly distributed in 1-3 500 bp.By comparing with GO,KEGG,COG,NR,Swiss-Prot,and Pfam databases,the full-length transcriptomes were annotated,of which 26 525 full-length transcriptomes were annotated in GO database.The KEGG database annotated 25 233 full-length transcriptomes.The COG database annotated 31 303 full-length transcriptomes.The NR database annotated 34 284 full-length transcriptomes.The Swiss-Prot database annotated 30 701 full-length transcriptomes.The Pfam database annotated 24 622 full-length transcriptomes.In addition,559 transcription factor,40 944 CDS,14 699 lncRNAs,7 types of SSR,28 423 AS,and 2 747 APA events were identified or predicted.【Conclusion】 This study obtained the full-length transcriptome sequencing data of SYFG of chicken,which improved the number and length of transcripts,perfected the functional annotation of transcripts,analyzed the type of AS and APA,and revealed the complexity of transcriptome,which could provide data support to further analyze the molecular regulatory mechanism of chicken follicular development from the transcriptome level.

Key words: Hy-Line variety Brown; granulosa cells of small yellow follicle(SYFG); the third generation sequencing; follicle development

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