肿节风三清颗粒安全性试验研究

doi:10.16431/j.cnki.1671-7236.2018.09.033

Abstract

Abstract:

The aim of this study was to investigate the acute toxicity,sub-chronic toxicity and target animal safety of Sarcandra glabra Sanqing granules,and provide theoretical basis for clinical safety medication.In the acute toxicity test,the mice were administrated once by gavage and the LD₅₀ was not detected.Therefore,the method of multiple doses administration within 24 h was conducted to determine the maximum tolerance dose.In the sub-chronic toxicity test,the rats were intragastrically administrated Sarcandra glabra Sanqing granules in three different doses of low,medium and high (5,10 and 20 g/(kg·BW)) once a day for 35 days and control group was set up.The clinical signs and weight changes of the rats were observed during the administration,on the 35th body weigh gained,blood examination,blood biochemical indicators,histopathological changes were observed respectively.In the safety test of target animal,the clinical recommended dose of 1,3 and 5 times were administered for continuous 5 d,the clinical signs of experimental chickens were observed and the blood and biochemical indicators were determined.As a result,in the acute toxicity test,mice were not dead in each dose group,the value of LD₅₀ was not measured,the maximum tolerance was 75 g/(kg·BW) (particle gauge).In the sub-chronic toxicity test,there was no significant difference in clinical signs,body weight,blood routine and blood biochemical indexes between the drug-administered rats and blank control rats (P>0.05).Histopathological observations revealed no abnormal lesions in the parenchymal organs.In the safety test of target animals,the weight gained,feed conversion rate,blood routine and biochemical indexes of chickens in each treatment group were not significantly different from those of blank control group (P>0.05).It indicated that the Sarcandra glabra Sanqing granules were actually non-acute toxicity and non-sub-chronic toxicity,and clinical use of Sarcandra glabra Sanqing granules was safe under the 5 times dose of the recommended dose for target animal.

Key words: Sarcandra glabra Sanqing granules; acute toxicity; sub-chronic toxicity; target animal safety

CLC Number:

S853.76

WEN Xuemei, LAI Shengji, HU Tingjun, CUI Yumei, LU Yanrong, ZHANG Xinqin, TANG Tingchong, YANG Shanzhong, HE Jiakang. Safety Study of Sarcandra glabra Sanqing Granules[J]. , 2018, 45(9): 2600-2609.

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Safety Study of Sarcandra glabra Sanqing Granules

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