地黄提取物的急性毒性和亚慢性毒性试验研究

doi:10.16431/j.cnki.1671-7236.2017.11.037

Abstract

Abstract:

The purpose of this experiment was to study the acute toxicity and long-term sub-chronic toxicity of the extract of Rehmannia glutinosa in mice, evaluate the clinical safety of medication and provide theoretical basis for clinical application. Mice were chosen to measure the median lethal dose (LD₅₀) and maximal tolerance dose (MTD). In the sub-chronic toxicity test, 80 SD rats were divided into four groups:Low dose, middle dose, high dose test groups with the extract of Rehmannia glutinosa and control group with normal saline for 30 d. On the 31th day, the rats were killed and the blood routine index, biochemistry index and the organ coefficient were measured. The MTD was 61.54 g/kg, according to the judgmental standard of the acute toxicity, the extract of Rehmannia glutinosa was safe. The sub-chronic toxicity test results showed that there were no significant difference in body weight and blood chemistry indexes and organ coefficient among the four groups (P>0.05) and there were no pathological change because of the medication. The result indicated that the extract of Rehmannia glutinosa was no acute toxicity under the condition of this test according to acute toxicity classification standard of exogenous by WTO, and it was no sub-chronic toxicity too, which suggested the extract had a good clinical safety.

Key words: extract of Rehmannia glutinosa; acute toxicity; sub-chronic toxicity; safety

CLC Number:

S859.7

LIU Jia, LI Qiang, GUO Li, LI Guo-hui, ZHAO Xing-hua, LIU Jing, HE Xin. Study on Acute Toxicity and Sub-chronic Toxicity of the Extract of Rehmannia glutinosa[J]. , 2017, 44(11): 3372-3378.

References

[1] 张兆顺,窦宝棠,白应利,等.抗生素饲料添加剂在畜牧业生产中过度使用的危害及对策[J].陕西农业科学,2012,3:127.
[2] Jian J C, Wu Z H. Influences of traditional Chinese medicine onnon-specific immunity of Jian carp (Cyprinus carpio var. Jian)[J]. Fish & Shellfish Immunology,2004, 2(16):185-191.
[3] Wang J L, Meng X L,Wu C, et al. Effects of Rehmannia glutinosa on growth performance, immunological parameters and disease resistance to Aeromonas hydrophila in common carp (Cyprinus carpio L.)[J]. Aquaculture, 2015, 1(435):293-300.
[4] Shi C J, Wen X S, Gao H F, et al. Steamed root of Rehmannia glutinosa Libosch. (Plantaginaceae) alleviates methotrexate-induced intestinal mucositis in rats[J].Journal of Ethnopharmacology, 2016, 183:143-150.
[5] 叶定江, 张世臣. 中药炮制学[M].北京:人民卫生出版社,1999.
[6] 李军,张丽萍,张振凌. 地黄研究进展[J].河南中医学院学报,2005,11(6):79-83.
[7] 李更生,于震,王慧森. 地黄化学成分与药理研究进展[J]. 国外医学中医中药分册,2004,26(2):74-77.
[8] 李红伟,孟祥乐. 地黄化学成分及其药理作用研究进展[J]. 药物研究评价,2015,38(2):218-228.
[9] Huang Y, Jiang C, Hu Y,et al. Immunoenhancement effect of Rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell[J]. Carbohydrate Polymers,2013,96(2):516-521.
[10] 王志江,魏国栋,马思缇. 地黄多糖的化学和药理作用研究进展[J]. 中国实验方剂学杂志, 2015, 21(16):231-235.
[11] 徐叔云, 卞如濂, 陈修.药理实验方法学[M]. 北京:人民卫生出版社, 2002.
[12] 吕安,张志聪,宫平.宫净灌注剂急性毒性和长期毒性试验研究[J]. 中国畜牧兽医,2016,43(8):2143-2149.
[13] 袁伯俊, 廖明旭, 李波. 药物毒理学实验方法与技术[M]. 北京:化学工业出版社,1997.
[14] 雷宏东,梁剑平,郭志廷. 常山提取物急性毒性试验研究[J]. 中国畜牧兽医,2011,38(6):236-238.
[15] 王东升, 谢家声, 李世宏, 等. 宫衣净酊急性毒性研究[J]. 湖北农业科学, 2011, 2:346-347.
[16] 离胜坤,王胜义,崔东安,等. 乌锦颗粒剂的急性毒性与亚慢性毒性试验研究[J]. 中国畜牧兽医,2015,42(5):1203-1210.
[17] 宋志勇,王蕊,张金凤,等. 地黄和胃口服液对小鼠的急性毒性试验[J]. 内蒙古中医药,2014,35:110.
[18] 董韡,徐晶,陈灵. 地黄中梓醇的提取鉴定及其急性毒性试验[J]. 复旦学报,2009,48(3):409-412.
[19] 姜玲玲,史开志,陈鸿宇. 一种新兽用止泻中药复方制剂对大鼠的急性及亚慢性毒性试验[J]. 中国畜牧兽医,2016,43(11):3073-3079.
[20] 苏亚楠,周君,鲍恩东,等. 糜蛋白酶的毒理试验[J]. 畜牧与兽医,2015, 47(9):95-98.
[21] 张玲,陆瑛,杨荣. SD大鼠血液及血清生化参考值的探讨[J]. 云南医药,2004,25(3):190-191.
[22] 吕圭源,陈素红,苏洁. 中药保健功能与优势[J]. 中国现代中药,2015,17(12):1241-1245.

Study on Acute Toxicity and Sub-chronic Toxicity of the Extract of Rehmannia glutinosa

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Yuanyuan, YANG Na, TENG Da, HAO Ya, MA Xuanxuan, MAO Ruoyu, WANG Jianhua. Preparation and Evaluation of Efficacy and Safety of Shengtaicin Post-medicated Bath Solution for Dairy Cow [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1617-1631.
[2]	ZHANG Wen, QIN Zhibiao, CHENG Jing, YU Meiling, WEI Yingyi, LI Jiang, HU Tingjun. Study on Acute and Subchronic Toxicity of Polygonum hydropiper L. Extract [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1532-1544.
[3]	QIAO Chunyu, LIU Jiahe, ZHANG Boxi, HE Yuxi, ZHENG Yuwei, LYU Hongming. Research Progress on Pathogenic Mechanism and Control of Aflatoxin B₁ [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 765-775.
[4]	CUI Yiyan, DENG Dun, TIAN Zhimei, LU Huijie, LIU Zhichang, LI Jiazhou, MA Xianyong, ZHANG Qi, LAN Yonghui. Research Progress on Nutritional Value and Feed Safety of Food Waste [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2820-2829.
[5]	XIANG Rong, LI Yahui, GAO Yuan, WEI Qilin, SHU Bingyao, LUO Shengjun. Study on Acute Toxicity and 30-day Feeding Test of Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz.Water Extract [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2262-2270.
[6]	GUO Yuli, LIU Junping, SUN Di, DONG Zhiying, WANG Peng, LIU Chenggong. Study on Toxicity and Safety Evaluation of Qinzhu Antai Powder [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3880-3888.
[7]	WEI Qianhan, LEI Weiwei, CHEN Xin, ZHAO Weiya, ZHANG Meng, XIAO Jinzha, ZHANG Yumei. Acute Toxicity and Subchronic Toxicity Test of Tribonema Powder [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1602-1610.
[8]	YANG Shuai, HU Xiangwei, ZHOU Di, ZHANG Xinxin, YANG Liguo. Study on the Growth Promoting Effect of Crossbred Buffalo Calves Immunized with DNA Vaccine of Co-expression of Somatostatin and Cortistatin [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4041-4050.
[9]	HE Mengru, ZHOU Ni, LYU Xiaohua. Risk Assessment of Bacitracin Resistance as a Medicated Feed Additive [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3749-3756.
[10]	WENG Jingyu, LIANG Liqing, ZHOU Jingkai, LIU Yuting, LI Li. Acute Toxicity and Hypoglycemic Activity of Total Flavonoids from Melastoma dodecandrum Lour. [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 275-281.
[11]	LIU Dingxi, YANG Jiecheng, ZHOU Hongchao, GUO Kangkang. Research Advance on Detection Methods of Antibacterial Residues in Animal Food [J]. , 2019, 46(7): 2183-2192.
[12]	ZENG Fanmei, CHONG Yuqing, JIANG Xunping, LIU Guiqiong. Research Progress on Immuno-castration in Livestock [J]. , 2019, 46(6): 1739-1745.
[13]	JIN Ye, LIANG Pengfei, FANG Mei, ZHANG Jianan, JIA Ning, DENG Kang, HUANG Weikuan. Extraction,Purification,Acute Toxicity and Antioxidant Activity of Total Flavonoids from Seeds of Ammopiptanthus [J]. , 2019, 46(3): 940-948.
[14]	SUN Jichao, DONG Zhen, CHEN Chen, ZHANG Jiyu, ZHOU Xuzheng. Experimental Study on Oral Acute Toxicity of Florfenicol Premix in Wistar Rats [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3785-3791.
[15]	LUO Lirong, WANG Hui, CUI Dongan, WANG Fuhe, WANG Shengyi. Study on Acute and Sub-chronic Toxicity Study of Zixiekang Oral Liquid [J]. , 2018, 45(9): 2582-2590.