China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (4): 1617-1631.doi: 10.16431/j.cnki.1671-7236.2023.04.033

• Basic Veterinary Medicine • Previous Articles     Next Articles

Preparation and Evaluation of Efficacy and Safety of Shengtaicin Post-medicated Bath Solution for Dairy Cow

LI Yuanyuan1,2, YANG Na1,2, TENG Da1,2, HAO Ya1,2, MA Xuanxuan1,2, MAO Ruoyu1,2, WANG Jianhua1,2   

  1. 1. Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Published:2023-04-06

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Abstract: 【Objective】 The purpose of the experiment was to prepare a new antibacterial peptide-Shengtaicin post-medicated bath solution,and characterize its effectiveness and safety.【Method】 The optimal formulation of Shengtaicin post-medicated bath solution was screened by one-way effect test and L9(3)3 orthogonal test.In vitro bactericidal test was used to characterize the bactericidal efficacy of the post-medicated bath solution.The safety was verified by hemolysis test,cytotoxicity test,skin irritation test in single-administered mice,skin irritation test in multiple-administered mice and skin sensitization test in mice.【Result】 The orthogonal test results showed that,the film solution was configured with 7% film-forming agent PVA1788 as the substrate,and glycerol had the greatest experimental impact,followed by Tween 80.The optimal formulation of the Shengtaicin post-medicated bath solution was as follow:The optimal addition volume fraction of the plasticizer glycerol was 4%,the optimal addition volume fraction of the surfactant Tween 80 was 1%,and the optimal addition mass percentage concentration of the thickener pullulan polysaccharide was 0.4%.More than 99.999% of the tested Staphylococcus aureus,Staphylococcus epidermidis,Streptococcus agalactiae and Streptococcus dysgalactiae were killed within 30 min.The bactericidal effect was comparable to that of povidone iodine.In addition,the hemolytic and cytotoxic properties of Shengtaicin post-medicated bath solution were lower than those of the post-bath solution of povidone-iodine.The results of single and multiple skin irritation test and skin sensitization test in mice showed that Shengtaicin post-medicated bath solution was non-irritating and non-sensitizing to the skin of mice.【Conclusion】 The antimicrobial peptide Shengtaicin post-medicated bath solution was a validity,green and safe bath,which could effectively prevent the occurrence of mastitis in cows,and it would ensure the quality and safety of raw milk and promote the healthy development of the dairy industry.

Key words: Shengtaicin; post-medicated bath solution; orthogonal design; efficacy; safety

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