丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响

doi:10.16431/j.cnki.1671-7236.2023.04.032

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effects of Clostridium butyrate (C.butyricum) and sodium butyrate on semen quality and testicular tissue of mice stimulated by high fat diet,so as to provide experimental basis and theoretical support for the treatment of male reproductive diseases.【Method】 In experiment 1,32 8-week-old C57BL/6 male mice were randomly divided into 4 groups.The mice in control group (NC) were fed a basal diet, in high fat diet group (HFD), C. butyricum treatment group (HFD+C.butyricum) and sodium butyrate treatment group (HFD+Sodium butyrate) were fed high fat diet containing 60% fat.HFD+C.butyricum group was gavaged with 1×10⁸ CFU/kg C.butyricum,while HFD+Sodium butyrate group was gavaged with 300 mg/kg sodium butyrate,once a day.In experiment 2,16 8-week-old C57BL/6 male mice were randomly divided into two groups.The normal diet of control group was supplemented with normal saline,and the experiment group was supplemented with C.butyricum solution 1×10⁸CFU/kg by gavage,once a day.After 12 weeks,the animals were sacrificed for sampling.The semen quality was detected by CASA,and the pathological changes of testicular tissue were observed by HE staining.The total antioxidant capacity (T-AOC),the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),the content of malondialdehyde (MDA) in testicular tissue and serum testosterone (T), and the activities of alkaline phosphatase (AKP),acid phosphatase (ACP),lactate dehydrogenase (LDH) and gamma glutamyltransferase (γ-GT) in testicular tissue were detected by corresponding kits.【Result】 In experiment 1,compared with high-fat group,the body weight of mice in C.butyricum treatment group was extremely significantly decreased (P＜0.01),the sperm motility,the number of A+B grade sperm,sperm linear motility and the velocity of average path of mice were significantly increased (P＜0.05),and the straight-line velocity of mice was extremely significantly increased (P＜0.01).The activity of AKP in testicular tissue of mice was significantly increased (P＜0.05).Compared with high-fat group,the body weight of mice in sodium butyrate treatment group was extremely significantly decreased (P＜0.01),the testis index,sperm linear motility,sperm forward motility,straight-line velocity and swing of sperm were significantly increased (P＜0.05),and the sperm motility,the number of A+B grade sperm,the velocity of average path and the activity of γ-GT in testicular tissue were extremely significantly increased (P＜0.01).In addition,sodium butyrate significantly increased the content of serum testosterone and the activity of ACP (P＜0.05),and significantly decreased the content of MDA in testicular tissue of mice (P＜0.05).The pathological section of testicular tissue showed that there was no significant difference in testicular tissue morphology of mice in each group.In experiment 2,compared with control group,the testicular coefficient of mice treated with C.butyricum were extremely significantly increased (P＜0.01),and the level of serum testosterone was significantly increased (P＜0.05),but there were no significant change in semen quality and antioxidant indexes of testicular tissue (P＞0.05).【Conclusion】 C.butyricum and sodium butyrate could significantly increase the content of serum testosterone in mice,improve the antioxidant capacity of testicular tissue,improve the quality and quantity of sperm,protect the structure of testicular tissue,and effectively protect the reproductive system injury caused by obesity in male mice.

Key words: Clostridium butyricum; sodium butyrate; high fat; testis; semen quality

CLC Number:

S816.73

DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.

References

[1] MOREIRA B P,MONTEIRO M P,SOUSA M,et al.Insights into leptin signaling and male reproductive health:The missing link between overweight and subfertility?[J].Biochemical Journal,2018,475(22):3535-3560.
[2] 郭向娇,李建彬,刘建勋,等.郑州市中老年居民超重和肥胖流行特征及影响因素分析[J].预防医学,2019,31(12):1245-1247. GUO X J,LI J B,LIU J X,et al.Epidemiological characteristics and influencing factors of overweight and obesity among middle-aged and elderly residents in Zhengzhou[J].Preventive Medicine,2019,31(12):1245-1247.(in Chinese)
[3] MÍNGUEZ-ALARCÓN L,CHAVARRO J E,MENDIOLA J,et al.Fatty acid intake in relation to reproductive hormones and testicular volume among young healthy men[J]. Asian Journal of Andrology,2017,19(2):184-190.
[4] CHAVARRO J E,MÍNGUEZ-ALARCÓN L,MENDIOLA J,et al.Trans fatty acid intake is inversely related to total sperm count in young healthy men[J].Human Reproduction,2014,29(3):429-440.
[5] JENSEN T K,HEITMANN B L,BLOMBERG J M,et al.High dietary intake of saturated fat is associated with reduced semen quality among 701 young Danish men from the general population[J].The American Journal of Clinical Nutrition,2013,97(2):411-418.
[6] RATO L,ALVES M G,CAVACO J E,et al.High-energy diets:A threat for male fertility?[J].Obesity Reviews,2014,15(12):996-1007.
[7] LANDRY D,CLOUTIER F,MARTIN L J.Implications of leptin in neuroendocrine regulation of male reproduction[J].Reproductive Biology,2013,13(1):1-14.
[8] 丁宁.肠道菌群失衡诱发雄性小鼠生精障碍的研究[D].广州:广东工业大学,2020. DING N.Study on the effect of intestinal microbiota imbalance on spermatogenesis in male mice[D].Guangzhou:Guangdong University of Technology,2020.(in Chinese)
[9] 李雄彪,马庆英,崔云龙.酪酸梭状芽胞杆菌研究进展[J].中国微生态学杂志,2006,4:331-335. LI X B,MA Q Y,CUI Y L.Research progress of Clostridium tyrosine[J].Chinese Journal of MicroEcology,2006,4:331-335.(in Chinese)
[10] 张善亭,史燕,张淑丽,等.丁酸梭菌的研究应用进展[J].生物技术通报,2013,9:27-33. ZHANG S T,SHI Y,ZHANG S L,et al.Advances in research and application of Clostridium butyricum[J].Biotechnology Bulletin,2013,9:27-33.(in Chinese)
[11] 张晓阳,卢忆,马艳莉,等.丁酸梭菌生理功能及应用研究进展[J].中国食物与营养,2012,18(12):31-35. ZHANG X Y,LU Y,MA Y L,et al.Research progress on physiological function and application of Clostridium butyricum[J]. Chinese Food and Nutrition,2012,18(12):31-35.(in Chinese)
[12] 黄潇航,张龙,胡奇林,等.丁酸梭菌作为饲料添加剂在动物养殖与疾病防控中的应用进展[J].中国饲料,2020,15:12-17. HUANG X H,ZHANG L,HU Q L,et al.Application of Clostridium butyricum as feed additive in animal breeding and disease control[J].China Feed Science,2020,15:12-17.(in Chinese)
[13] 谢树贵,戴青,赵述淼,等.丁酸梭菌对动物致病菌的拮抗作用研究[J].湖北农业科学,2007,3:424-426. XIE S G,DAI Q,ZHAO S M,et al.Antagonism of Clostridium butyricum against animal pathogens[J].Hubei Agricultural Sciences,2007,3:424-426.(in Chinese)
[14] 杜云平,周庆丰,郑泽铭,等.日粮中添加丁酸梭菌对肉鸡生产性能的影响[J].广东饲料,2009,18(11):22-23. DU Y P,ZHOU Q F,ZHENG Z M,et al.Effects of dietary Clostridium butyricum on broiler performance[J]. Guangdong Feed,2009,18(11):22-23.(in Chinese)
[15] 林华林,秦颖超,周加义,等.丁酸梭菌及其代谢产物对畜禽肠道健康影响的研究进展[J].饲料工业,2019,40(6):7-12. LIN H L,QIN Y C,ZHOU J Y,et al.Research progress on effects of Clostridium butyricum and its metabolites on intestinal health of livestock and poultry[J].Feed Industry,2019,40(6):7-12.(in Chinese)
[16] HUANG J,CAI J,WANG J,et al.Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor[J].Bioresour Technol,2011,102(4):3923-3926.
[17] GUILLOTEAU P,MARTIN L,EECKHAUT V,et al.From the gut to the peripheral tissues:The multiple effects of butyrate[J].Nutrition Research Reviews,2010,23(2):366-384.
[18] 徐振丽,陈计峦,裴龙英,等.奶酪风味及芳香物质研究进展[J].中国调味品,2019,44(12):181-184. XU Z L,CHEN J L,PEI L Y,et al.Research progress of cheese flavor and aromatic substances[J].Chinese Condiments,2019,44(12):181-184.(in Chinese)
[19] 王金丹,乔莞宁,朱静,等.酪酸梭菌代谢产物丁酸和氢气对急性胃黏膜损伤的作用[J].中国病理生理杂志,2017,33(10):1906-1911. WANG J D,QIAO W N,ZHU J,et al.Effects of Clostridium butyrate and hydrogen on acute gastric mucosal injury[J].Chinese Journal of Pathophysiology,2017,33(10):1906-1911.(in Chinese)
[20] HUDCOVIC T,KOLINSKA J,KLEPETAR J,et al.Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis:Differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice[J].Clinical and Experimental Immunology,2012,167(2):356-365.
[21] 尚海啸.丁酸梭菌对于高脂膳食诱导的小鼠肥胖和结肠免疫紊乱的调节作用[D].无锡:江南大学,2016. SHANG H X.The effect of Clostridium butyricum on obesity and colonic immune disorder induced by high-fat diet in mice[D].Wuxi:Jiangnan University,2016.(in Chinese)
[22] YAN W J,MU Y,YU N,et al.Protective effects of metformin on reproductive function in obese male rats induced by high-fat diet[J]. Journal of Assisted Reproduction and Genetics,2015,32(7):1097-1104.
[23] YI X,GAO H,CHEN D,et al.Effects of obesity and exercise on testicular leptin signal transduction and testosterone biosynthesis in male mice[J].American Journal of Physiology Regulatory,Integrative and Comparative Physiology,2017,312(4):R501-R510.
[24] CAMPOS-SILVA P,FURRIEL A,COSTA W S,et al.Metabolic and testicular effects of the long-term administration of different high-fat diets in adult rats[J]. International Brazjurol,2015,41(3):569-575.
[25] 曹利,赵杰,陈铃霞,等.玉米赤霉烯酮与脱氧雪腐镰刀菌烯醇联合染毒对雄性小鼠生殖毒性的影响及茶多酚的缓解作用[J].动物营养学报,2020,32(4):1946-1956. CAO L,ZHAO J,CHEN L X,et al.Effects of combined exposure of zearalenone and deoxynivalenol on reproductive toxicity of male mice and alleviating effects of tea polyphenols[J].Chinese Journal of Animal Nutrition,2020,32(4):1946-1956.(in Chinese)
[26] 杨贤树,吴方超,张翔栋,等.环境污染物对种公畜精子质量影响的研究进展[J].黑龙江畜牧兽医,2021,19:44-49. YANG X S,WU F C,ZHANG X D,et al.Research progress on effects of environmental pollutants on sperm quality of breeding stock[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2021,19:44-49.(in Chinese)
[27] 伏彭辉,张家庆,毛献宝,等.不同品种公猪精子活率及运动能力的比较[J].黑龙江畜牧兽医,2010,5:53-55. FU P H,ZHANG J Q,MAO X B,et al.Comparison of sperm motility and motility of different breeds of boars[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2010,5:53-55.(in Chinese)
[28] MOLINE J M,GOLDEN A L,BAR-CHAMA N,et al.Exposure to hazardous substances and male reproductive health:A research framework[J].Environmental Health Perspectives,2000,108(9):803-813.
[29] RICHBURG J H.The relevance of spontaneous- and chemically-induced alterations in testicular germ cell apoptosis to toxicology[J].Toxicology Letters,2000,112-113:79-86.
[30] TIAN X,YU Z,FENG P,et al. Lactobacillus plantarum TW1-1 alleviates diethylhexylphthalate-induced testicular damage in mice by modulating gut microbiota and decreasing inflammation[J].Frontiers in Cellular and Infection Microbiology,2019,9:221.
[31] 王希春,曹丽,刘秦,等.脱氧雪腐镰刀菌烯醇和黄曲霉毒素B₁单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量[J].浙江农业学报,2019,31(6):886-892. WANG X C,CAO L,LIU Q,et al.Effects of single and combined exposure of deoxynifuenol and aflatoxin B₁ on pathological changes,antioxidant properties and tight junction protein expression in mouse brain tissue[J].Journal of Zhejiang Agricultural Sciences,2019,31(6):886-892.(in Chinese)
[32] 黄洋,郭青枝,郭春燕,等.维生素D对发生氧化损伤的猪睾丸间质细胞保护作用的研究[J].黑龙江畜牧兽医,2022,5:16-19. HUANG Y,GUO Q Z,GUO C Y,et al.Protective effect of vitamin D on mesenchymal cells of porcine testis[J].Heilongjiang Animal Husbandry and Veterinary Science,2022,5:16-19.(in Chinese)
[33] 杨小军,贺喜,何丽霞,等.日粮添加多不饱和脂肪酸对肉仔鸡抗氧化指标的影响[J].动物营养学报,2008,20(3):299-304. YANG X J,HE X,HE L X,et al.Effects of dietary polyunsaturated fatty acids on antioxidant indices of broilers[J].Chinese Journal of Animal Nutrition,2008,20(3):299-304.(in Chinese)
[34] UTOMO A,JIANG X,FURUTA S,et al.Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells[J].The Journal of Biological Chemistry,2004,279(42):43522-43529.
[35] 索川,雷治海,程玲,等.短期热量限制对雄性大鼠睾丸形态和抗氧化能力的影响[J].畜牧与兽医,2017,49(6):66-71. SUO C,LEI Z H,CHENG L,et al.Effects of short-term caloric restriction on testicular morphology and antioxidant capacity in male rats[J].Animal Science & Veterinary Medicine,2017,49(6):66-71.(in Chinese)
[36] 金亚倩,刘文忠,赵俊星,等.酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响[J].畜牧兽医学报,2016,47(11):2228-2239. JIN Y Q,LIU W Z,ZHAO J X,et al.Effects of wine grape skin residue on reproductive performance and testis antioxidant activity of RAMS fed in single column[J].Chinese Journal of Animal Science and Veterinary Medicine,2016,47(11):2228-2239.(in Chinese)
[37] 王卫杰,李惠,王乐,等.饲料添加剂的蓄积对幼犬睾丸功能相关酶活性的影响[J].饲料博览,2016,7:1-3. WANG W J,LI H,WANG L,et al.Effects of feed additive accumulation on the activities of testicular function-related enzymes in puppies[J]. Feed Review,2016,7:1-3.(in Chinese)
[38] 张波,徐光翠,刘峰,等.氧乐果对小鼠睾丸特征性酶的影响及茶多酚的拮抗作用[J].环境与健康杂志,2005,22(5):340-342. ZHANG B,XU G C,LIU F,et al.Effects of oxomethoate on characteristic enzymes of mouse testis and antagonistic effects of tea polyphenols[J]. Journal of Environment and Health,2005,22(5):340-342.(in Chinese)
[39] GENG X,SHAO H,ZHANG Z,et al.Malathion-induced testicular toxicity is associated with spermatogenic apoptosis and alterations in testicular enzymes and hormone levels in male Wistar rats[J]. Environmental Toxicology and Pharmacology,2015,39(2):659-667.
[40] 郭海,才秀莲,王国秀.锰诱导大鼠生精细胞凋亡过程中半胱氨酸酶-3 mRNA与增殖细胞核抗原表达变化[J].解剖学杂志,2015,38(1):15-17. GUO H,CAI X L,WANG G X.Expression of cysteinase-3 mRNA and proliferating cell nuclear antigen in manganese-induced apoptosis of rat spermatogenic cells[J].Chinese Journal of Anatomy,2015,38(1):15-17.(in Chinese)
[41] 李晓凤,杨旸,周义军.妊娠期氰戊菊酯暴露对雄性仔鼠睾丸标志酶的影响[J].上海交通大学学报(医学版),2011,31(8):1117-1120. LI X F,YANG Y,ZHOU Y J.Effects of prenatal fenvalerate exposure on testicular marker enzymes in male offspring mice[J]. Journal of Shanghai Jiao Tong University (Medical Edition),2011,31(8):1117-1120.(in Chinese)
[42] LONG M,YANG S,DONG S,et al.Characterization of semen quality,testicular marker enzyme activities and gene expression changes in the blood testis barrier of Kunming mice following acute exposure to zearalenone[J].Environ Science and Pollution Research International,2017,24(35):27235-27243.
[43] 许颖洁,郑朱安贞,诸天源,等.纳米硒铈联合作用对微波辐射小鼠雄性生殖功能的影响[J].卫生研究,2020,49(5):795-801. XU Y J,ZHENG Z A J,ZHU T Y,et al.Effects of selenium-cerium nanoparticles on male reproductive function in mice exposed to microwave radiation[J]. Health Research,2020,49(5):795-801.(in Chinese)
[44] HAMMES S R,LEVIN E R.Impact of estrogens in males and androgens in females[J]. The Journal of Clinical Investigation,2019,129(5):1818-1826.
[45] 林勇,何成添,范碧霞,等.躁狂患者血清睾酮水平与暴力行为倾向及认同的相关性[J].临床精神医学杂志,2021,31(2):127-129. LIN Y,HE C T,FAN B X,et al.Correlation between serum testosterone level and violent behavior tendency and identification in manic patients[J].Journal of Clinical Psychiatry,2021,31(2):127-129.(in Chinese)
[46] WALKER W H.Testosterone signaling and the regulation of spermatogenesis[J].Spermatogenesis,2011,1(2):116-120.
[47] NIU S,CHEN J,DUAN F,et al.Possible mechanism underlying the effect of Heshouwuyin,a tonifying kidney herb,on sperm quality in aging rats[J].BMC Complementary and Alternative Medicine,2014,14:250.
[48] 阳田恬,刘梦瑶,张静,等.丁酸梭菌的特性及其生物学功能[J].饲料工业,2022,43(6):59-64. YANG T T,LIU M Y,ZHANG J,et al.Characteristics and biological functions of Clostridium butyricum[J].Feed Industry,2022,43(6):59-64.(in Chinese)
[49] RODEN A C,MOSER M T,TRI S D,et al.Augmentation of T cell levels and responses induced by androgen deprivation[J].Journal of Immunology,2004,173(10):6098-6108.
[50] 李玉鹏,李海花,李义海,等.丁酸梭菌对不同遗传背景仔猪TLR2信号通路差异调控的分子机制[J].黑龙江畜牧兽医,2021,21:8-14. LI Y P,LI H H,LI Y H,et al.Molecular mechanism of Clostridium butyricum on TLR2 signaling pathway in piglets with different genetic backgrounds[J]. Heilongjiang Animal Husbandry and Veterinary Science,2021,21:8-14.(in Chinese)
[51] TREMELLEN K,MCPHEE N,PEARCE K,et al.Endotoxin-initiated inflammation reduces testosterone production in men of reproductive age[J].American Journal of Physiology Endocrinology and Metabolism,2018,314(3):E206-E213.
[52] 舒均兰,徐娥,桂国弘,等.丁酸梭菌早期干预对大肠杆菌攻毒肉仔鸡肠道菌群和短链脂肪酸的影响[J].饲料工业,2021,42(11):44-52. SHU J L,XU E,GUI G H,et al.Effects of early intervention of Clostridium butyricum on intestinal flora and short chain fatty acids in broilers challenged with E. coli[J].Feed Industry,2021,42(11):44-52.(in Chinese)
[53] LI D,LIU R,WANG M,et al.3β-hydroxysteroid dehydrogenase expressed by gut microbes degrades testosterone and is linked to depression in males[J]. Cell Host Microbe,2022,30(3):329-339.e5.
[54] 张凌寒.幽门螺杆菌感染与雄鼠睾酮及氧化应激水平的相关性研究[D].郑州:郑州大学,2021. ZHANG L H.Study on the correlation between Helicobacter pylori infection and testosterone and oxidative stress in male rats[D].Zhengzhou:Zhengzhou University,2021.(in Chinese)
[55] POUTAHIDIS T,SPRINGER A,LEVKOVICH T,et al.Probiotic microbes sustain youthful serum testosterone levels and testicular size in aging mice[J]. PLoS One,2014,9(1):e84877.

Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SONG Chenglei, ZHANG Zhe, WEI Yilin, YANG Songbai, DOU Yaqing, LI Chenlei, LIU Yingke, LI Xinjian, YANG Feng, LI Xiuling, WANG Kejun, QIAO Ruimin, HAN Xuelei. Effects of Different Breeds,Seasons and Month Ages on Semen Quality of Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1938-1946.
[2]	LIU Zhen, ZHOU Xueqin, XU Fei, LI Zhefeng, SUN Hanxue, LI Guoqin, TAO Zhengrong, GUAN Mei, ZHENG Qiang, DIAO Xinping, LU Lizhi. Effects of Coated Sodium Butyrate on Growing Performance,Slaughter Performance, Immune Organ Index and Digestive Enzyme Activity of Breeding Pigeons and Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 998-1006.
[3]	CHEN Jing, YU Shuyi, HUANG Hui, LI Jianzhe. Effect of Clostridium butyrate on the Composition of Plasma Free Fatty Acid in Mice Fed with High Choline Diet [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 519-530.
[4]	ZHANG Xiaoke, LIAO Weili, LI Tingting, LI Yao, LI Jiaqi, HUANG Xiang, ZHANG Hao. Effects of Semen Collectors and Environmental Parameters in Pigpen on Semen Quality of Duroc Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 220-229.
[5]	WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang. Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3453-3464.
[6]	ZHANG Yuzhen, HUANG Minyi, CHEN Kechun, CAI Yongming, YANG Yiqi. Effects of High Fat and High Fructose Diet on Retinal Morphology in Aged Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2515-2522.
[7]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[8]	XU Xiaoling, BAI Jiahua, XIAO Linli, QIN Yusheng, SONG Yuqing, LIU Yan. Effects of Compound Additives on the Quality and Conception Rate of Dairy Cows Thawed Sex Controlled Frozen Semen [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2654-2660.
[9]	HU Wen, HUO Konglin, SONG Xingxing, ZHANG Xin, ZHANG Duoni, LUO Rongrong, XU Wenhao, LI Xun. Effects of Gonadotropin Inhibitory Hormone on Gonadal Reproductive Function and Glucose Metabolism in SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2239-2249.
[10]	YUAN Chongshan, WANG Jun, LYU Wenfa. Research Progress on Effects of Polyunsaturated Fatty Acids on Animal Semen Quality [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1422-1429.
[11]	LUO Lan, CUI Chao, DONG Ruilan, LIU Haoyu, LIU Xiaobin, YU Guanghui. Effects of Different Concentrations of Rutin and Freezing Rates on Cryopreservation of Boar Semen [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 982-991.
[12]	HU Xiaobing, LIN Biaosheng, WANG Zhenwei, ZHENG Lijing. Isolation, Identification and Genomic Sequence Analysis of a Strain of Clostridium butyricum from Pigs Utilizing Lactic Acid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 569-578.
[13]	CHEN Jiakang, LYU Chunrong, MIAO Yongwang, QUAN Guobo. Research Progress on Application of Lipidomic Technology in Quality Assessment and in vitro Storage of Livestock Semen [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4697-4706.
[14]	SHAN Chunqiao, ZHAI Hongxu, LI Juan, WANG Yan, YU Hongmin, WU Yiqi, LIU En, LIU Yan. Effects of Clostridium butyricum on Intestinal Short-chain Fatty Acid Content and Microflora Diversity of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4239-4251.
[15]	CHU Xu, ZHANG Junxia, WANG Jing. Synergistic Effect of Four Chinese Herbal Monomers and Sodium Butyrate on the Expression of Porcine Host Defense Peptide [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4063-4074.